Blood

	or
Pink top tube 6 mL (K2-EDTA)		Lavender top tube 3 mL (EDTA)

Adults - 2 mL
Pediatrics - 1 mL or EDTA Microtainer^®

Specimen must be labeled with patient's first and last name and medical record number. Specimens will be rejected if information is not on the label when received.

24 hrs/day, 7 days a week, including holidays.

2 hours (upon receipt in laboratory)

Negative result means that no antibodies were detected on the patient's red cells using polyspecific antiglobulin or anti IgG, depending on the patient age. If the patient is greater than four months old, poly specific antiglobulin is used. If the patient is less than four months old, only IgG is used.

Monospecific testing for IgG and C3 complement is automatically performed when the polyspecific test is positive.

Elution performed per pathologist recommendation or clinician order.

Only monospecific testing of IgG will be performed on cord samples when mothers are alloimmunized, when mother's antibody status is unknown, or on samples from patients < 4 months old.

Tube test

If specimen is received in the laboratory greater than 24 hours from time of collection and has not been refrigerated, it will be rejected.

Invert tube gently several times to mix blood.
Label transport tube with patient last name, first name, identification number, date and time of collection.
Do Not Centrifuge.
Submit whole blood in original container.

Room temperature or refrigerate if stored overnight.

Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Place specimen into Styrofoam container, seal container.
Ship at room temperature.