Peripheral Blood, Bone Marrow, or CSF

Yellow top tube 8.5 mL (ACD solution A)

Green top tube 4 mL (Na Heparin) or Lavender top tube 3 mL (EDTA)

Adult - Peripheral Blood: 10 mL; Bone Marrow:  2-4 mL

Pediatric - Peripheral Blood:  2-5 mL; Bone Marrow:  2-5 mL

CSF - Volume required is cell count dependent.  Body fluid specimens 
containing small number of leukocytes may not be adequate for 
immunophenotyping. Provide as much CSF specimen as possible in the 
original CSF collection tube(s).

0800-1630 Monday through Friday.

2 days

Antibodies routinely included are: CD1A, CD2, CD3, CD4, CD5, CD7, 
CD8, CD10, CD11B, CD13, CD14, CD15, CD16, CD19, CD20, CD22, CD33, 
CD34, CD36, CD38, CD45, CD56, CD61, CD64, CD71, CD117, HLA-DR, Kappa, 
Lambda.

Additional antibodies are added as necessary for diagnosis after 
morphologic examination. The pathologist will provide an 
interpretative report.

Epic code: LAB8255 for Blood, LAB8284 for Bone Marrow, LAB8254 for Other

For further details, refer to How to Send Flow Cytometry Specimens.

If after hours service is needed, contact UIHC operator at 319-356-1616 and have the Pathology Resident on-call paged.

Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Comprehensive Hematopathology Requisition before shipping the specimen. Include pertinent clinical information on the requisition.

Additional antibodies may be necessary for diagnosis.

Recent corticosteroid or chemotherapy may invalidate result.

Flow Cytometry-Lysed Whole Blood/Marrow

Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as possible.

Room Temperature.

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place requisition into outside pocket of bag.
Ship at room temperature.
Recommend early AM overnight shipping or equivalent if not on courier service.