Cholesterol, High-Density Lipoprotein

Label Mnemonic: HDLP
Epic code: LAB101
Order form: Laboratory Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 83718
Specimen(s):
Plasma
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:

3 mL whole blood from light green top tube or 1 Microtainer® for pediatric patients

Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:

Guidelines from the National Lipid Association and the National Cholesterol Education Program for cardiovascular risk for adults 18 years of age and older:

 

Males: 40 mg/dL or higher

Females: 50 mg/dL or higher

 

The Expert Panel on Integrated Guidelines for Cardiovascular Health and Risk Reduction in Children and Adolescents has set the following guidelines for lipids in a context of cardiovascular risk for children 2-17 years of age:

 

HDL CHOLESTEROL

Low HDL: <40 mg/dL

Borderline Low: 40-45 mg/dL

Acceptable: >45 mg/dL

 

Reference values have not been established for patients who are younger than 24 months of age.

Comments:

Fasting for at least 8 hours prior to collection is recommended.

 

Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Laboratory Requisition before shipping the specimen.

 

The Expert Panel on Integrated Guidelines for Cardiovascular Health and Risk Reduction in Children and Adolescents has set the following guidelines for lipids in a context of cardiovascular risk for children 2-17 years of age:

 

HDL CHOLESTEROL

Low HDL: <40 mg/dL

Borderline Low: 40-45 mg/dL

Acceptable: >45 mg/dL

 

Reference values have not been established for patients who are younger than 24 months of age.

 

References

  1. Jacobson TA, Ito MK, Maki KC, et al: National Lipid Association recommendations for patient-centered management of dyslipidemia: Part 1-executive summary. J Clin Lipidol. 2014;8(5):473-488. doi: 10.1016/j.jacl.2014.07.007

  2. Expert Panel on Integrated Guidelines for Cardiovascular Health and Risk Reduction in Children and Adolescents; National Heart, Lung, and Blood Institute: Expert panel on integrated guidelines for cardiovascular health and risk reduction in children and adolescents: Summary report. Pediatrics. 2011 Dec;128 Suppl 5(Suppl 5):S213-S256. doi: 10.1542/peds.2009-2107C
Test Limitations:
Criterion: Recovery within plus or minus 10% of initial value.

Icterus: No significant interference up to an I index of 60.

Hemolysis: No significant interference up to an H index of 1200 
(approximate hemoglobin concentration: 1200 mg/dL).

Lipemia (Intralipid): No significant interference up to an L index of 
2000. There is poor correlation between turbidity and triglycerides 
concentration.

Ascorbic acid: No significant interference up to 50 mg/dL.

In rare cases, elevated immunoglobulin concentrations can cause 
unreliable results.  Abnormal liver function does affect lipid 
metabolism; consequently, HDL and LDL results are of limited 
diagnostic value. In some patients with abnormal liver function, the 
HDL result may significantly differ from the DCM (designated 
comparison method) result.

No interference was found at therapeutic concentrations using common 
drug panels. Statins and fibrates tested at therapeutic concentration 
ranges did not interfere.  N-acetylcysteine at the therapeutic 
concentration when used as an antidote and the acetaminophen 
metabolite N-acetyl-p-benzoquinone imine (NAPQI) independently may 
cause falsely low results.

Venipuncture immediately after or during the administration of 
metamizole may lead to falsely low results.
Methodology:

Enzymatic

Assay updated to Roche HDL Generation 4 on 06/04/18

Sample Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Sample Storage:
Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
 
See Additional Information:
Fasting Specimen Requirements