Large Granular Lymphocytic Leukemia (LGL) Flow Cytometry Panel

Epic code: See comments
Order form: Comprehensive Hematopathology Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 88184,
88185 x 15,
88189
Specimen(s):
Various
Collection Medium:
Yellow top tube 8.5 mL (ACD solution A)
Alternate Collection Media:
Green top tube 4 mL (Na Heparin) or Lavender top tube 3 mL (EDTA)
Minimum:

Peripheral Blood: 2 mL

 

Bone Marrow: 2–4 mL yellow top tube (ACD solution A)

 

Fluids and Tissue: Dispense sample into vial of RPMI-1640 tissue culture media.

Testing Schedule:

Samples will be processed during the hours of 0800–1600 daily. Samples that arrive on weekends or holidays will be triaged and processed during the next business day. For additional services, contact Clinical Pathology Resident on-call at pager #3404

Turn Around Time:
2 days
Reference Range:

If malignancy is detected the pathologist will add tests on as needed.

Comments:

LAB8255 for Blood, LAB8284 for Bone Marrow, LAB8254 for Other

For further details, refer to How to Send Flow Cytometry Specimens.

Please state the clinical question to be answered on the requisition. Maintain the specimen at room temperature; do not refrigerate or incubate.

Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Comprehensive Hematopathology Requisition before shipping the specimen.

 

Antibodies: CD3, CD4, CD5, CD7, CD8, CD10, CD19, CD20, CD23, CD45, CD56, CD57, CD200, Monoclonal Kappa, Monoclonal Lambda, FMC7

Methodology:

Flow Cytometry-Lysed Whole

Sample Processing:
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as possible.
Sample Storage:
Room Temperature.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at room temperature.
Recommend early AM overnight shipping or equivalent if not on courier service.