Duffy (Fya/Fyb) Antigen Phenotype

Label Mnemonic: DUFFY
Epic code: LAB9538
Order form: DeGowin Blood Center Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 86905
Specimen(s):
Blood
Collection Medium:
or or
Pink top tube 6 mL (K2-EDTA) Lavender top tube 3 mL (EDTA) Lavender top tube 0.5 mL (EDTA)
Minimum:

Adults (18 years and older): Full 6mL Pink top tube

Pediatrics (1 year through 17 years old): Full 3mL Lavender top tube

Pediatrics (4 months up to 1 year old): 1mL in a 3mL Lavender tube 

Neonates (0 - < 4 months old): 0.5 mL in Lavender Microtainer®

 

If these age/container guidelines are not followed, it will result in a rejected sample and a new one will have to be collected.

Rejection Criteria:

Specimen must be labeled with patient’s first and last name and medical record number.  Specimens will be rejected if information is not on the label when received.

Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
24 hours upon receipt at reference laboratory (upon receipt in laboratory)
Reference Range:

Red cell antigens are tested with antisera to determine phenotype.

Comments:

Duffy antigen testing is useful to identify patients at risk for Duffy-Null Associated Neutrophil Count (DANC).  Patients negative for both FyA and FyB are considered at risk for DANC.

Methodology:

Tube test

Instructions:

If specimen will be received in the laboratory within 24 hours of collection, refrigeration is not required.

Sample Processing:
Invert tube gently several times to mix blood.
Label transport tube with patient last name, first name, identification number, date and time of collection.
Do Not Centrifuge.
Submit whole blood in original container.
Sample Storage:
Room temperature or refrigerate if stored overnight.
Transport Instructions:
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Place specimen into Styrofoam container, seal container.
Ship at room temperature.