Tumor Gene RNA Fusion with Interpretation
Label Mnemonic: | CTL-TUMOR |
Epic code: | LAB8956 |
Order form: | Molecular Oncology Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 81445 |
Specimen(s):
Formalin-fixed, paraffin embedded tissue block, or ten unstained 4
micron-thick sections on non-plus slides and one H&E stained section
Minimum:
One tumor-containing formalin-fixed paraffin embedded tissue block or 10 unstained 4 micron-thick sections on non-plus slides and one H&E stained section from one tumor-containing FFPET block or any or all unstained or stained slides from tumor-containing fine needle aspirate cytologic preparations.
Rejection Criteria:
Specimens that have been decalcified will not be accepted.
Turn Around
Time:
10-14 days
Reference Range:
Fusion or mutation not detected
Comments:
Tissue sections are reviewed by a pathologist. RNA is isolated from
the selected area of the sample. Anchored multiplex PCR for targeted
next-generation sequencing is performed. The sequenced sample is a
reverse transcription PCR-amplified fragment library in which each
sample is uniquely identified by ligation of a short oligonucleotide
barcode. The resultant sequence identifies the exons of the fusion
transcript arising from the specific gene target and the partner
gene.
The panel targets gene rearrangements of these gene targets: AKT1, ALK, AXL, BRAF, CCND1, EGFR (vIII), FGFR1, FGFR2, FGFR3, MET (exon 14 skip), NRG1, NTRK1, NTRK2, NTRK3, PPARG, RAF1, RET, ROS, and THADA.
The panel targets gene rearrangements of these gene targets: AKT1, ALK, AXL, BRAF, CCND1, EGFR (vIII), FGFR1, FGFR2, FGFR3, MET (exon 14 skip), NRG1, NTRK1, NTRK2, NTRK3, PPARG, RAF1, RET, ROS, and THADA.
Methodology:
Massively parallel next generation sequencing on RNA
Sample
Storage:
Room Temperature.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship at room temperature.
Protect block or slides from excessive heat and ship in cooled container during summer months.
Place requisition into outside pocket of bag.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship at room temperature.
Protect block or slides from excessive heat and ship in cooled container during summer months.
CPT Code:
81445