Microsporidia Species, PCR
| Label Mnemonic: | LCMSP |
| Epic code: | LAB8918 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 87798 |
Specimen(s):
Stool
Collection Medium:
)
|
| Sterile Container |
Minimum:
Preferred Minimum: 5 g of stool in clean unpreserved stool transport
vial.
Absolute Minimum: 1 g of stool
Absolute Minimum: 1 g of stool
Testing Schedule:
Monday through Saturday
Turn Around Time:
2 days upon receipt at reference laboratory
Reference Range:
Negative
Interpretive Data:
A positive result indicates the presence of
Enterocytozoon bieneusi and
Encephalitozoon species DNA and is consistent with an
active or recent infection. Since microsporidia DNA may be present
in stool or urine in the absence of clinical symptoms, results
should be correlated with clinical presentation.
A negative result indicates absence of detectable DNA from Enterocytozoon bieneusi and Encephalitozoon species in the specimen, but does not always rule out ongoing microsporidiosis since the organism may be present at very low levels or may be sporadic.
Other tests to consider in the evaluation of a patient presenting with acute or chronic watery diarrhea include cultures or specific assays for bacterial, viral, and parasitic pathogens.
A negative result indicates absence of detectable DNA from Enterocytozoon bieneusi and Encephalitozoon species in the specimen, but does not always rule out ongoing microsporidiosis since the organism may be present at very low levels or may be sporadic.
Other tests to consider in the evaluation of a patient presenting with acute or chronic watery diarrhea include cultures or specific assays for bacterial, viral, and parasitic pathogens.
Comments:
Useful for the detection of (em>Enterocytozoon bieneusi and
Encephalitozoon species in stool and urine specimens to
support the clinical diagnosis of microsporidiosis.
Cautions:
This test only detects DNA from Enterocytozoon bieneusi and Encephalitozoon species and does not detect the less common microsporidia. These 2 genera are the most common causes of intestinal and renal microsporidiosis.
While this assay is designed to detect symptomatic infection with Enterocytozoon bieneusi and Encephalitozoon species, it may detect asymptomatic carriage and should only be used for patients with a clinical history and symptoms consistent with microsporidiosis.
Cautions:
This test only detects DNA from Enterocytozoon bieneusi and Encephalitozoon species and does not detect the less common microsporidia. These 2 genera are the most common causes of intestinal and renal microsporidiosis.
While this assay is designed to detect symptomatic infection with Enterocytozoon bieneusi and Encephalitozoon species, it may detect asymptomatic carriage and should only be used for patients with a clinical history and symptoms consistent with microsporidiosis.
Methodology:
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Instructions:
Refrigerate within 3 hours of collection.
Sample Processing:
Label transport tube with two patient identifiers, date and time of
collection.
Keep refrigerated and submit to laboratory.
Keep refrigerated and submit to laboratory.
Transport Instructions:
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Ship refrigerated.
Place specimen into zip-lock type bag, seal bag.
Ship refrigerated.