Cancer Mutation Profiling Blood Paired Normal Testing

Label Mnemonic:	CHPNML
Epic code:	LAB8080
Order form:	Molecular Oncology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	81445

Specimen(s):

Blood

Collection Medium:

Pink top tube 6 mL (K2-EDTA)

Minimum:

Collect blood in Pink top tube.

Testing Schedule:

Weekly

Turn Around Time:

21 days

Comments:

The Cancer Mutation Profiling test is a massively parallel next generation DNA sequencing-based assay that targets commonly mutated or hotspot regions of 50 cancer-related genes. Some testing requires comparison to the patient's germline sequence and a peripheral blood specimen will usually be sufficient.

Genes Assessed: ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MET, MLH1, MPL, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARC1, SMO, SRC, STK11, TP53, and VHL.

Methodology:

Massively parallel Next Generation DNA sequencing by synthesis, semiconductor technology, Ion Personal Genomics Machine

Sample Storage:

Refrigerate.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship refrigerated.
Place requisition into outside pocket of bag.