Bilirubin, Direct
| Label Mnemonic: | BILD |
| Epic code: | LAB52 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 82248 |
Specimen(s):
Plasma
Collection Medium:
 |
| Plasma Separator Tube 4.5 mL |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood from light green top tube or ONE light green Microtainer® for pediatric patients
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
1 hour (upon receipt in laboratory)
Reference Range:
0.0 - 0.2 mg/dL
Comments:
Avoid hemolysis and unnecessary exposure to light.
Test
Limitations:
Hemolysis leads to decreased direct bilirubin concentrations. Direct bilirubin will not be reported for specimens with severe enough hemolysis to significantly impact results (40 – 150 mg/dL hemoglobin depending on direct bilirubin concentration).
No significant interference up to lipemia levels greater than 750 mg/dL (Intralipid).
Methodology:
Spectrophotometric
Assay updated to Roche cobas® Bilirubin Direct Gen.2 on 10/08/2024.
Instructions:
Avoid hemolysis and unnecessary exposure to light.
Sample
Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Sample
Storage:
Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.