EGFR Gene Analysis with Interpretation

Label Mnemonic:	EGFR
Epic code:	LAB4821
Order form:	Molecular Oncology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	81235

Specimen(s):

Formalin fixed, paraffin embedded tissue block and 10 unstained 5-10 μM slides at 20-25°C and an H&E slide of the tissue

Minimum:

Tumor cells more than 50% of the total tissue and greater than 10mm² in surface area on the block

Rejection Criteria:

Specimens fixed in B5 fixative or that have been decalcified will not be accepted. Tumor specimens containing less than 50% tumor cells or are less than 10mm² in area may be unacceptable.

Testing Schedule:

Weekly

Turn Around Time:

7-10 working days

Reference Range:

Negative

Methodology:

PCR followed by DNA Sequencing

Instructions:

Submit formalin fixed, paraffin embedded tissue block or 10 unstained
5-10 μM slides and an H&E slide along with the requisition. Please include surgical pathology report and relevant clinical information.

Sample Processing:

Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Formalin-fixed, paraffin embedded tissue should be submitted.

Sample Storage:

Tissue Specimen - Freeze immediately and store frozen or send Formalin-fixed paraffin embedded blocks.

Transport Instructions:

Frozen Tissue - Ship frozen on dry ice. Ship express mail with overnight delivery. Formalin-fixed paraffin embedded blocks ship at room temperature.
Place requisition into outside pocket of bag.
Protect block or slides from excessive heat and ship in cooled container during summer months.