JAK2 Common Mutation V617F Analysis with Interpretation
Label Mnemonic: | JAK2 |
Epic code: | LAB2472 |
Order form: | Comprehensive Hematopathology Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 81279 |
Specimen(s):
Whole Blood
Collection Medium:
Pink top tube 6 mL (K2-EDTA) |
Minimum:
Adults 3 mL whole blood. Testing on smaller volumes than those
requested will be attempted. However, in some cases, small blood
volumes may compromise the ability to perform testing. Testing
requires a dedicated collection tube.
Testing
Schedule:
Weekly
Turn Around
Time:
7 working days
Reference Range:
Direct detection of G-T transversion at position 1849 of the Janus
kinase 2 locus, giving rise to a Valine-to-Phenylalanine substitution
at position 617 (JAK2 V617F mutation).
Comments:
This test is useful for diagnosis of myeloproliferative disorders:
Polycythemia Vera and Essential Thrombocythemia.
Methodology:
Allele specific real-time Polymerase Chain Reaction
Sample
Processing:
Label transport tube with two patient identifiers, date and time of collection.
Patient's age and sex is required on requisition for processing.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Lavender tube is sent as whole blood, no processing necessary.
Do Not Centrifuge.
Patient's age and sex is required on requisition for processing.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Lavender tube is sent as whole blood, no processing necessary.
Do Not Centrifuge.
Sample
Storage:
Room temperature for up to 24 hours, then refrigerate the whole blood if it is necessary to be held overnight, weekends, or holidays.
Transport
Instructions:
Place requisition into outside pocket of bag.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship refrigerated.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship refrigerated.
CPT Code:
81279