TRG Gene Clonality by PCR with Interpretation

Label Mnemonic:	TCLBLD, TCLBM, TRGGE
Epic code:	LAB7360 for Blood, LAB8634 for Bone Marrow, LAB2487 for Tissue
Order form:	Molecular Oncology Requisition Comprehensive Hematopathology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	81342

Specimen(s):

Blood, Bone Marrow and Tissue

Collection Medium:

Pink top tube 6 mL (K2-EDTA)

Minimum:

5 mL blood in a pink top, 1 mL bone marrow aspirate in a pink top, 3 cu mm of fresh frozen tissue or formalin-fixed, paraffin embedded tissue, body fluids in a pink top or lymph node aspirates in RPMI.

Testing Schedule:

Weekly

Turn Around Time:

7 working days

Reference Range:

None

Comments:

DNA extracted from bone marrow mononuclear cells or tissue is examined for rearrangement of T-cell receptor gamma (TCRG) genes.

Test Limitations:

Specimens fixed in B5 fixative are unsuitable for testing.

Methodology:

Multiplex Polymerase Chain Reaction (PCR) followed by Fluorescence Capillary Electrophoresis

Sample Processing:

Label transport tube with two patient identifiers, date and time of collection.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen source is required on requisition for processing.
Tissue specimen should be frozen.

Sample Storage:

Blood/Bone Marrow - Room temperature or ambient storage.
Tissue Specimen - Freeze immediately and store frozen or send Formalin-fixed paraffin embedded blocks.

Transport Instructions:

Blood - Do not freeze, ship at room temperature. Ship express mail with overnight delivery.
Frozen Tissue - Ship frozen on dry ice. Ship express mail with overnight delivery. Formalin-fixed paraffin embedded blocks ship at room temperature.