Fluorescence In-Situ Hybridization (FISH-Aneuploidy Screening)

Label Mnemonic:	ANEUP
Order form:	Constitutional Genetics Pre and Post-Natal Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	88271 x5, 88275 x5, 88291

Specimen(s):

Paraffin Embedded Tumor Tissue or Peripheral Blood (Newborn or Cord, and Others)

Minimum:

4 sections cut at 2 microns and an accompanying H&E side from the tissue block with the most villi.

2 cc of infants venous blood collected in a green-top Vacutainer^® with sodium heparin. Invert tube to mix well. Label the tube with patient name and medical record number. DO NOT FREEZE OR CENTRIFUGE.

Testing Schedule:

Specimens accepted in the lab Monday-Friday, 0800-1700.

Turn Around Time:

Preliminary results are given for STAT blood cases within 48 hours. Suitable for interphase and metaphase analysis. Allow 2-5 days for final results. Preliminary results are not provided for paraffin embedded tumor tissue samples.

Reference Range:

Male:  X and Y probe signals          Female:  two X probe signals
The reference range for autosomes varies for each probe, call lab for 
information.

Comments:

Epic code: CYT54

Shivanand R. Patil Cytogenetics & Molecular Laboratory Website

Methodology:

Fluorescence In-Situ Hybridization (FISH)

Instructions:

Submit specimen to laboratory as soon as possible after collection. Provide details of clinical information and family history (if applicable). If a specimen is collected over the weekend, please call (319) 356-1616 and ask the operator to page the cytogenetics on-call staff at pager #5525.

For billing and/or shipping questions, contact University of Iowa Diagnostic Laboratories at 1-866-844-2522 (toll free) or 319-384-7213 (Fax).

Sample Processing:

Do Not Centrifuge.
Maintain sterility and forward promptly to lab.

Sample Storage:

Specimen cannot be frozen.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Ship at room temperature.