Varicella Zoster IgG Detection
| Label Mnemonic: | VZSC |
| Epic code: | LAB2437 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 86787 |
Specimen(s):
Plasma
Collection Medium:
|
| Plasma Separator Tube 4.5 mL |
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection
containers.
Minimum:
3.0 mL whole blood from light green top tube or TWO Microtainer®
devices.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
3 hours (upon receipt in laboratory)
Reference Range:
Reference range and methodology changed effective 12/11/2012.
0.8 AI or less: Negative - No significant level of detectable varicella- zoster IgG antibody.
0.9-1.0 AI: Equivocal - Repeat testing in 10-14 days may be helpful.
1.1 AI or greater: Positive - IgG antibody to varicella-zoster detected, which may indicate a current or past exposure/immunization to varicella-zoster.
0.8 AI or less: Negative - No significant level of detectable varicella- zoster IgG antibody.
0.9-1.0 AI: Equivocal - Repeat testing in 10-14 days may be helpful.
1.1 AI or greater: Positive - IgG antibody to varicella-zoster detected, which may indicate a current or past exposure/immunization to varicella-zoster.
Interpretive Data:
The best evidence for current infection is a significant change on
two appropriately timed specimens, where both tests are done in the
same laboratory at the same time.
Varicella-zoster IgG antibody can be formed following varicella-zoster infection or after varicella-zoster vaccination. A reactive result is consistent with immune status to varicella-zoster virus. Non-reactive and equivocal results flag as abnormal in Epic which indicates non- immune or equivocal immune status to varicella-zoster. A non-reactive result does NOT imply varicella-zoster infection. If ordered in workup of possible varicella-zoster infection, the IgG antibody results should be interpreted in conjunction with other laboratory tests, clinical history, and physical examination.
Varicella-zoster IgG antibody can be formed following varicella-zoster infection or after varicella-zoster vaccination. A reactive result is consistent with immune status to varicella-zoster virus. Non-reactive and equivocal results flag as abnormal in Epic which indicates non- immune or equivocal immune status to varicella-zoster. A non-reactive result does NOT imply varicella-zoster infection. If ordered in workup of possible varicella-zoster infection, the IgG antibody results should be interpreted in conjunction with other laboratory tests, clinical history, and physical examination.
Test Limitations:
Patients with a current primary infection of varicella may not begin
producing measurable concentrations of anti- varicella antibodies
until several days after infection. An assay for the presence of IgM
varicella antibodies may be helpful in diagnosing recent
infection.
A positive test result in immunocompromised patients receiving prophylactic treatment with zoster immune serum globulin or zoster immune plasma may not be indicative of prior infection with VZV.
A positive test result in immunocompromised patients receiving prophylactic treatment with zoster immune serum globulin or zoster immune plasma may not be indicative of prior infection with VZV.
Methodology:
Multiplex Flow Immunoassay
Sample Processing:
Centrifuge at a speed and time necessary to get barrier separation
of plasma/serum and cells.
Separate plasma or serum into labeled container and cap.
Separate plasma or serum into labeled container and cap.
Sample Storage:
Refrigerate.
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal
bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.