Reticulocyte Count (Automated)

Label Mnemonic:	ARET
Epic code:	LAB296
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	85045

Specimen(s):

Whole Blood

Collection Medium:

Lavender top tube 3 mL (EDTA)

Minimum:

Full draw; 3.0 mL lavender top; (or fingerstick-purple top microtainer^®)

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

1 hour (upon receipt in laboratory)

Reference Range:

Age                     Absolute Reticulocyte Range
      1 - 3 Days*            77 - 283 K/mm3
      4 - 30 Days*           14 - 159 K/mm3
     31 - 60 Days*           28 - 201 K/mm3
60 Days - Adult              12 - 130 K/mm3
*Full Term Infant

% Reticulocytes:  0.4 - 2.1%

Comments:

Refer to BD Microtainer^® Tubes product sheet for detailed sample collection instructions.

A reticulocyte count can usually be added to any CBC sample <24 hours old.

Methodology:

Flow Cytometry
Reticulocyte counts are performed using flow cytometry method.  The 
RNA/DNA in the Reticulocytes is stained and the stained cells are then 
detected and enumerated on the basis of a two-dimensional distribution 
of the forward scattered light and lateral fluorescent light.

Sample Processing:

Lavender tube is sent as whole blood, no processing necessary.

Sample Storage:

Refrigerate, 2-8°C.

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.