Reticulocyte Count (Automated)
Label Mnemonic: | ARET |
Epic code: | LAB296 |
Order form: | Laboratory Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 85045 |
Specimen(s):
Whole Blood
Collection Medium:
Lavender top tube 3 mL (EDTA) |
Minimum:
Full draw; 3.0 mL lavender top; (or fingerstick-purple top
microtainer®)
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
1 hour (upon receipt in laboratory)
Reference Range:
Age Absolute Reticulocyte Range 1 - 3 Days* 77 - 283 K/mm3 4 - 30 Days* 14 - 159 K/mm3 31 - 60 Days* 28 - 201 K/mm3 60 Days - Adult 12 - 130 K/mm3 *Full Term Infant % Reticulocytes: 0.4 - 2.1%
Comments:
Refer to BD Microtainer® Tubes product sheet for detailed sample collection
instructions.
A reticulocyte count can usually be added to any CBC sample <24 hours old.
A reticulocyte count can usually be added to any CBC sample <24 hours old.
Methodology:
Flow Cytometry Reticulocyte counts are performed using flow cytometry method. The RNA/DNA in the Reticulocytes is stained and the stained cells are then detected and enumerated on the basis of a two-dimensional distribution of the forward scattered light and lateral fluorescent light.
Sample
Processing:
Lavender tube is sent as whole blood, no processing necessary.
Sample
Storage:
Refrigerate, 2-8°C.
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
85045