Glomerular Basement Membrane Antibodies, IgG
| Label Mnemonic: | GBM |
| Epic code: | LAB1255 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 83520 |
Specimen(s):
Serum
Collection Medium:
|
| Red top tube 5 mL (Clot Activator) |
Minimum:
Adult - 5 mL; red top tube Pediatric - 2 mL; red top tube
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
3 hours (upon receipt in laboratory)
Reference Range:
Negative: < 0.4 antibody index (AI)
Equivocal: 0.4-0.9
Positive: 1.0 AI or greater
Equivocal: 0.4-0.9
Positive: 1.0 AI or greater
Interpretive Data:
Glomerular basement membrane (GBM) antibodies are used for
evaluating with rapid onset renal failure or pulmonary hemorrhage
and as an aid in the diagnosis of Goodpasture syndrome in
conjunction with clinical history and physical examination. Positive
results for GBM antibodies are consistent with Goodpasture syndrome.
GBM antibodies are highly specific for Goodpasture syndrome. The
sensitivity of this test is approximately 87% in untreated patients
with systemic disease.
Comments:
Assay methodology and reference ranges changed February 5, 2013.
References:
Pusey CD. Anti-glomerular basement membrane disease. Kidney Int 2003; 64:1535-1550.
References:
Pusey CD. Anti-glomerular basement membrane disease. Kidney Int 2003; 64:1535-1550.
Methodology:
Multiplex flow immunoassay
Sample Processing:
Centrifuge at a speed and time necessary to get barrier separation
of plasma/serum and cells.
Separate plasma or serum into labeled container and cap.
Separate plasma or serum into labeled container and cap.
Sample Storage:
Refrigerate.
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal
bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.