Lymphocyte Subsets

Order form:	Comprehensive Hematopathology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	88184 x1, 88185 x5 Technical, 88187 Professional

Specimen(s):

Peripheral Blood

Collection Medium:

Yellow top tube 8.5 mL (ACD solution A)

Alternate Collection Media:

Lavender top tube 3 mL (EDTA)

Minimum:

Adult: 8.5 mL whole blood in a yellow top tube (ACD solution A)
Pediatric: 2 mL whole blood in a lavender top tube (EDTA)

Testing Schedule:

0800-1630 Monday through Friday.

Turn Around Time:

2 days

Reference Range:

The pathologist will provide an interpretative report.  
Antibodies routinely included are:  CD3, CD4, CD8, CD16/56, CD19, and 
CD45.

Adult reference ranges for peripheral blood by whole blood lysis 
method using flow cytometry:

B cells (CD19)                       6-22%
T cells (CD3)                       65-85%
T helper/induce (CD4+/CD3+)         30-62%
T cytotoxic/suppressor (CD8+/CD3+)  13-37%
NK cells (CD16+/CD56+/CD3-)         5-31%
CD3+/CD16+/CD56+                    0-6%
CD4/CD8 ratio                       0.7-2.7

Age specific pediatric reference ranges will be provided with the 
interpretive report.

Comments:

Epic code: LAB8255

For further details, refer to How to Send Flow Cytometry Specimens.

Methodology:

Flow Cytometry-Whole Blood Lysis

Sample Processing:

Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as possible.

Sample Storage:

Room Temperature.

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Ship at room temperature.
Recommend early AM overnight shipping or equivalent if not on courier service.