Fetal Erythrocyte Quantitation

Label Mnemonic:	FHGB
Epic code:	LAB292

Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009 or 467-6010
Tube Station #260

Specimen(s):

Maternal Peripheral Blood

Specimen Instructions:

Specimen should be ambient or room temperature.

Collection Medium:

Lavender top tube 3 mL (EDTA)

Minimum:

1 mL whole blood

Rejection Criteria:

Cord blood samples are NOT accepted.

Delivery Instructions:

Deliver to laboratory immediately after collection. Send to tube station #260 or deliver to Specimen Control, 6240 RCP.

Testing Schedule:

0800-1630 Monday through Friday. For additional services, contact Clinical Pathology Resident on-call at pager #3404.

Turn Around Time:

24 hours (upon receipt in laboratory)

Reference Range:

Reference range is less than 0.3%
Positive specimens reported as percent of maternal cells.

The normal "Hemoglobin F value" for non-pregnant adults is less than
0.1%.

Note: This reference range is established as the level at which 
greater than the usual 300 micrograms dose administered to Rh-negative 
women at delivery is required to prevent sensitization.

                      % HbF by Flow     Total RhoGAM (vials)
                         < 0.30%                 1
                       0.3% - 0.89%              2
                       0.9% - 1.49%              3
                       1.5% - 2.09%              4
                       2.1% - 2.69%              5
                          >=2.7%        Contact DBC Faculty

Comments:

Please identify as MATERNAL or FETAL specimen. Screening test for
fetal-maternal bleed.

Optimal results are obtained from a sample within 6 hours of 
venipuncture.  This procedure may obtain results from a sample up to 
30 hours from collection.

Call Flow Cytometry (467-6010) if testing is required on a fetal 
sample.

Methodology:

Flow Cytometry

CPT Code:

88184

See Additional Information:
Specimens Requiring Immediate Delivery