Vitamin B12
| Label Mnemonic: | B12 |
| Epic code: | LAB67 |
| Downtime form: | Doctor/Provider Orders - Pathology Core and Specialty Care Nursery |
Chemistry
6240 RCP
356-3527
6240 RCP
356-3527
Specimen(s):
Plasma
Collection Medium:
 |
| Plasma Separator Tube 4.5 mL |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in light green top tube or TWO
Microtainer® devices.
Delivery Instructions:
Deliver to laboratory immediately after collection.Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
1 hour (upon receipt in laboratory)
Reference Range:
Normal: 232 - 1245 pg/mL
Indeterminate: 150 - 231 pg/mL
Deficient: is less than 150 pg/mL
Indeterminate: 150 - 231 pg/mL
Deficient: is less than 150 pg/mL
Comments:
This assay may be significantly impacted by high-dose biotin (>5 mg
dose) taken within previous 12 hours. High concentrations of biotin
may lead to falsely increased results. These concentrations may be
found in patients taking over-the-counter supplements with biotin
content much higher than nutritional requirements for biotin.
Specimens should not be collected until at least 12 hours after the
last dose.
Refer to BD Microtainer® Tubes product sheet for detailed sample collection instructions.
New analytical immunoassay with different reference range instituted on 12/19/2017.
This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).
Refer to BD Microtainer® Tubes product sheet for detailed sample collection instructions.
New analytical immunoassay with different reference range instituted on 12/19/2017.
This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).
Test
Limitations:
The assay is unaffected by icterus (bilirubin is less than 65 mg/dL),
hemolysis (Hb is less than 1.0 g/dL), lipemia (triglycerides is less
than 1500 mg/dL) and biotin is less than 50 ng/mL. (criterion:
recovery within plus or minus 10% of initial value).
No interference was observed from rheumatoid factors up to a concentration of 1500 IU/mL.
In vitro tests were performed on 16 commonly used pharmaceuticals. No interference with the assay was found.
In rare cases interference due to extremely high titers of antibodies to streptavidin and ruthenium can occur.
This assay is designed to avoid interference due to anti-intrinsic factor antibodies.
For diagnostic purposes, the vitamin B12 findings should always be assessed in conjunction with the patient's medical history, clinical examination and other findings.
No interference was observed from rheumatoid factors up to a concentration of 1500 IU/mL.
In vitro tests were performed on 16 commonly used pharmaceuticals. No interference with the assay was found.
In rare cases interference due to extremely high titers of antibodies to streptavidin and ruthenium can occur.
This assay is designed to avoid interference due to anti-intrinsic factor antibodies.
For diagnostic purposes, the vitamin B12 findings should always be assessed in conjunction with the patient's medical history, clinical examination and other findings.
Methodology:
Electrochemiluminescence Immunoassay
CPT Code:
82607
See Additional Information:
Biotin Interference with Immunoassays
Specimens Requiring Immediate Delivery
Biotin Interference with Immunoassays
Specimens Requiring Immediate Delivery