Acute Leukemia Flow Cytometry Panel

Epic code: See comments
Order form: Epic Beaker AP Order Label
Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009 or 467-6010
Tube Station #260
Specimen(s):
Peripheral Blood, Bone Marrow, or CSF
Collection Medium:
Yellow top tube 8.5 mL (ACD solution A)
Alternate Collection Media:
Green top tube 4 mL (Na Heparin) or Lavender top tube 3 mL (EDTA)
Minimum:

Adult - Peripheral Blood: 10 mL; Bone Marrow: 2-4 mL

 

Pediatric - Peripheral Blood: 2-5 mL; Bone Marrow: 2-5 mL

 

CSF - Volume required is cell count dependent. Body fluid specimens containing small number of leukocytes may not be adequate for immunophenotyping. Provide as much CSF specimen as possible in the original CSF collection tube(s).

Delivery Instructions:
Keep at room temperature. Do not refrigerate.

Send to tube station #260 or deliver to Specimen Control, 6240 RCP.

Testing Schedule:

Samples will be processed during the hours of 0800–1600 daily. Samples that arrive on weekends or holidays will be triaged and processed during the next business day. For additional services, contact Clinical Pathology Resident on-call at pager #3404.

Turn Around Time:
2 days
Comments:

Epic code: LAB8255 for Blood, LAB8284 for Bone Marrow, LAB8254 for Other

 

Include pertinent clinical information on the requisition.

 

Additional antibodies may be necessary for diagnosis.

 

Antibodies routinely included are: CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11B, CD13, CD14, CD15, CD16, CD19, CD20, CD22, CD33, CD34, CD36, CD38, CD45, CD56, CD64, CD71, CD117, CD123, HLA-DR, Kappa, Lambda.

 

Additional antibodies are added as necessary for diagnosis after morphologic examination. The pathologist will provide an interpretative report.

Test Limitations:

Recent corticosteroid or chemotherapy may invalidate result.

Methodology:

Flow Cytometry-Lysed Whole Blood/Marrow

CPT Code:
Technical: 88184 (first antibody), 88185 (each additional antibody); Professional: 88189
 
See Additional Information:
Specimens Requiring Immediate Delivery