LDH Isoenzymes

Label Mnemonic:	LDISO
Epic code:	LAB3487
Downtime form:	Doctor/Provider Orders - Pathology Core and Specialty Care Nursery

Commercial Mailout Laboratory
6240-8 RCP
356-8593

Specimen(s):

Serum

Collection Medium:

Red top tube 5 mL (Clot Activator)

Minimum:

Preferred Minimum: 1 mL serum
Absolute Minimum: 0.6 mL serum

Rejection Criteria:

Specimens collected with EDTA, potassium oxalate, or sodium fluoride anticoagulants. Frozen, refrigerated, or hemolyzed specimens.

Turn Around Time:

2 working days upon receipt at reference laboratory

Reference Range:

Components                     Reference Interval
LD-1                           14-27% of total
LD-2                           29-42% of total
LD-3                           18-30% of total
LD-4                            8-15% of total
LD-5                            6-23% of total
Lactate Dehydrogenase, Total   0 up to 30 days:  200-465 U/L
                               30 days-17 mos:   200-450 U/L
                               18 mos-10 yrs:    165-430 U/L
                               11 yrs-16 yrs:    127-287 U/L
                               17 yrs & over:    105-230 U/L

Comments:

Note: LD-1 and LD-2 are elevated in hemolyzed specimens and serum which has not been separated from cells. LD-3, LD-4, and LD-5 are labile at low temperatures, and are erroneously low in specimens that have been refrigerated or frozen.

Methodology:

Enzymatic/Electrophoresis

CPT Code:

83615 LD Total, 83625 LD Isoenzyme