Arbovirus Antibodies, IgG and IgM

Label Mnemonic:	ARBOGM
Epic code:	LAB8728
Downtime form:	Doctor/Provider Orders - Pathology Core and Specialty Care Nursery

Commercial Mailout Laboratory
6240-8 RCP
356-8593

Specimen(s):

Serum

Specimen Instructions:

Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as "acute" or "convalescent."

Collection Medium:

Red top tube 5 mL (Clot Activator)

Minimum:

Preferred Minimum: 1 mL serum in red top tube
Absolute Minimum: 0.5 mL serum in red top tube

Rejection Criteria:

Contaminated, hemolyzed, or severely lipemic specimens.

Turn Around Time:

1-5 days upon receipt at reference laboratory

Reference Range:

Components                       Reference Interval
St. Louis Encephalitis           Less than 1:16
  Antibody, IgG by IFA, Serum

California Encephalitis          Less than 1:16
  Antibody, IgG by IFA, Serum

Eastern Equine Encephalitis      Less than 1:16
  Antibody, IgG by IFA, Serum

Western Equine Encephalitis      Less than 1:16
  Antibody, IgG by IFA, Serum

West Nile Virus Antibody,        1.29 IV or less: Negative - No 
  IgG by ELISA, Serum            significant level of West Nile virus
                                 IgG antibody detected
                                 1.30-1.49 IV: Equivocal - Questionable
                                 presence of West Nile virus IgG
                                 antibody detected.  Repeat testing in
                                 10-14 days may be helpful.
                                 1.50 IV or greater: Positive -
                                 Presence of IgG antibody to West Nile
                                 virus detected, suggestive of current
                                 or past infection.

St. Louis Encephalitis           Less than 1:16
  Antibody, IgM by IFA, Serum

California Encephalitis          Less than 1:16
  Antibody, IgM by IFA, Serum

Eastern Equine Encephalitis      Less than 1:16
  Antibody, IgM by IFA, Serum

Western Equine Encephalitis      Less than 1:16
  Antibody, IgM by IFA, Serum

West Nile Virus Antibody,        0.89 IV or less: Negative - No 
  IgM by ELISA, Serum            significant level of West Nile virus
                                 IgM antibody detected.
                                 0.90-1.10 IV: Equivocal -
                                 Questionable presence of West Nile
                                 virus IgM antibody detected.  Repeat
                                 testing in 10-14 days may be helpful.
                                 1.11 IV or greater: Positive -
                                 Presence of IgM antibody to West Nile
                                 virus detected, suggestive of current
                                 or recent infection.

Interpretive Data:

Refer to report.

Comments:

This test is recommended as an aid in the diagnosis of infection with arboviruses.

Methodology:

Semi-Quantitative Indirect Fluorescent Antibody/Semi-Quantitative Enzyme-Linked Immunosorbent Assay

CPT Code:

86651 x2; 86652 x2; 86653 x2; 86654 x2; 86789; 86788