Lymphocyte Subsets Flow Cytometry Panel

Label Mnemonic:	TBNK
Epic code:	LAB9383
Order form:	Epic Beaker AP Order Label

Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009 or 467-6010
Tube Station #260

Specimen(s):

Peripheral Blood

Collection Medium:

Lavender top tube 3 mL (EDTA)

Alternate Collection Media:

Pink top tube 6 mL (K2-EDTA)

Minimum:

Adult: 3 mL whole blood in a purple top tube (EDTA)
Pediatric: 2 mL whole blood in a lavender top tube (EDTA)

Delivery Instructions:

Keep at room temperature. Do not refrigerate.

Send to tube station #260 or deliver to Specimen Control, 6240 RCP. Deliver ASAP, sample stability is 24 hours

Testing Schedule:

Samples are batched and will be processed during the hours of 0800–1600 daily. Sample stability is 24 hours, samples older than 24 hours will be cancelled as unacceptable. Samples that arrive on weekends or holidays will be triaged and processed during the next business day. For additional services, contact Clinical Pathology Resident on-call at pager #3404

Turn Around Time:

24 hours (upon receipt in laboratory)

Reference Range:

The pathologist will provide an interpretative report.  

Adult reference ranges for peripheral blood by whole blood lysis 
method using flow cytometry:

B cells (CD19)                      6-22%
T cells (CD3)                       65-85%
T helper/induce (CD4+/CD3+)         30-62%
T cytotoxic/suppressor (CD8+/CD3+)  13-37%
NK cells (CD16+/CD56+/CD3-)         5-31%
CD3+/CD16+/CD56+                    0-6%
CD4/CD8 ratio                       0.7-2.7

Age specific pediatric reference ranges will be provided with the 
interpretive report.

Comments:

Antibodies routinely included are: CD3, CD4, CD8, CD16/56, CD19, and CD45.

Methodology:

Flow Cytometry-Whole Blood Lysis

CPT Code:

88184, 88185 x5 Technical, 88187 Professional

See Additional Information:
Specimens Requiring Immediate Delivery