How to maximize your success in identifying glomeruli.
If able to confidently identify glomeruli as described above, one 3mm piece for IF and one 1mm piece for EM is all that is required for tissue adequacy. If using the "blind technique" more tissue will be needed obviously.
Two cores of tissue
a. Light Microscopy (LM):
b. Immunofluorescent (IF) Specimen:
c. Electron Microscopy:
One core samples: