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Flow Cytometry Service
Sergei Syrbu MD PhD, Director
                                     
A national anatomic and clinical pathology reference laboratory
affiliated with UI Health Care

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Department of Pathology



10 Color Flow Cytometry Services



The Immunopathology Laboratory performs several categories of diagnostic testing including autoimmune disease serologies, flow cytometry, immunohistochemistry, and immunofluorescence microscopy.

Autoimmune disease serologies include screening for diseases such as systemic lupus erythematosus (SLE), Sjogren's syndrome, scleroderma, Wegner's granulomatosis and related vasculitides, autoimmune hepatitis, primary biliary cirrhosis, ulcerative colitis, celiac disease and others. Testing methodologies used include enzyme immunoassay direct and indirect immunofluorescence microscopy.

Flow cytometry is done on bone marrow, peripheral blood, fine needle aspirations, miscellaneous body fluids, lymph nodes and solid tumors. Flow cytometry has a wide range of uses from typing lymphomas and leukemias to quantitation of lymphocyte subsets in suspected immunodeficiency diseases.

Ten color Flow Cytometry employs both gating and cluster analysis stratgies to maximize the sensitiveity and accuracy of the testing.

Immunohistochemistry staining is done primarily on formalin-fixed, paraffin embedded tissues using automated staining equipment. A large number of antibodies are available to immunophenotype and identify the source of tumors and to detect certain infectious agents (particularly viruses) in tissue sections. In-situ hybridization studies are also performed to identify certain types on infections, particularly Epstein-Barr virus.

Immunofluorescence microscopy is done on frozen sections of the skin, mucosae and the kidney to diagnose autoimmune diseases.


Turnaround Time: Routine timeframe for turnaround is 2-5 days.
In many cases a preliminary result can be given on the day the specimen arrives.

Lab Hours: Monday through Friday 8:00 am - 5:00 pm
Weekend and Holiday STAT service available as needed.

Requisition Form: Please print, fully complete, and send an Flow Cytometry Requisition Form with your specimens.

Anatomic Pathology consultation services: Flow Cytometry Consultation Overview

Sergei I. Syrbu, MD, PhD
Director
319-356-7372
sergi-syrbu@uiowa.edu
Carol J. Holman, MD, PhD
Associate Professor
carol-holman@uiowa.edu
319-356-3981
Michael S. Icardi, MD
Associate Professor
319-384-8632
michael-icardi@uiowa.edu
Nitin J. Karandikar, MD, PhD
Professor
319-384-9609
nitin-karandikar@uiowa.edu
John D. Kemp, MD
Professor
john-kemp@uiowa.edu
319-384-9611
Prerna Rastogi, MD, PhD
Assistant Professor
prerna-rastogi@uiowa.edu
319-356-3781
Mary Sue Otis, MBA
UIDL Business Manager
319-356-3533
marysue-otis@uiowa.edu
UIDL Client Services
866-844-2522
Lisa Horning, MT(ASCP)SI
Lead Scientist
319-356-2846
lisa-horning@uiowa.edu