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Flow Cytometry Service
Sergei Syrbu MD PhD, Michael S. Icardi, MD, and John D. Kemp, MD
A national anatomic and clinical pathology reference laboratory
affiliated with UI Health Care

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Department of Pathology

Flow Cytometry Service

The Immunopathology Laboratory performs several categories of diagnostic testing including autoimmune disease serologies, flow cytometry, immunohistochemistry, immunofluorescence microscopy, immunofixation and high-resolution agarose electrophoresis.

Autoimmune disease serologies include screening for diseases such as systemic lupus erythematosus (SLE), Sjogren's syndrome, scleroderma, Wegner's granulomatosis and related vasculitides, autoimmune hepatitis, primary biliary cirrhosis, ulcerative colitis, celiac disease and others. Testing methodologies used include enzyme immunoassay, indirect immunofluorescence microscopy, and immunodiffusion.

Flow cytometry is done on bone marrow, peripheral blood, fine needle aspirations, miscellaneous body fluids, lymph nodes and tumors. Flow cytometry has a wide range of uses from typing lymphomas and leukemias to quantitation of lymphocyte subsets in suspected immunodeficiency diseases.

Immunohistochemistry staining is done primarily on formalin-fixed, paraffin embedded tissues using automated staining equipment. A large number of antibodies are available to immunophenotype and identify the source of tumors and to detect certain infectious agents (particularly viruses) in tissue sections. In-situ hybridization studies are also performed to identify certain types on infections, particularly Epstein-Barr virus.

Immunofluorescence microscopy is done on frozen sections of skin, kidney, lymph node, and tumors. It is used both to diagnose autoimmune diseases of the skin, mucosae and the kidney and to immunophenotype lymphoid proliferations and several other types of tumors.

Lab Hours: Monday through Friday 8:00 am - 5:00 pm

Requisition Form: Please print, fully complete, and send an Flow Cytometry Requisition Form with your specimens.

Anatomic Pathology consultation services: Flow Cytometry Consultation Overview

Immunopathology Director
More information is avaibable by contacting
Sergei I. Syrbu, MD, PhD
Immunopathology Faculty
More information is avaibable by contacting
Michael S. Icardi, MD
Immunopathology Faculty
More information is avaibable by contacting
John D. Kemp, MD
UIDL Business Manager
Mary Sue Otis
Client Services
Lead Scientist
Lisa Horning