Histology Research Laboratory

The Histology Research Laboratory provides services for the processing, embedding, sectioning, and tinctorial and immunohistochemical staining of tissues. Expertise in the handling of fresh, frozen, and fixed cells and tissues is the building block of the laboratory.

The laboratory primarily serves the faculty and staff of the Department of Pathology, but is available to provide services for a fee to outside investigators.

If you would be interested in utilizing the sevices of the Histology Research Laboratory, please contact Christine Hochstedler, 319-335-8145 and/or fill out the Work Request Form. You can send the form either via email or bring it with your samples to the lab.

List of Services:

Tissue Processing:

Processing - To include specimen (fresh, frozen, fixed) handling, prep, mounting, machine processing, administrative, clerical, overhead.

Embedding

Sectioning/Cuts - One cut is the sample material placed onto a slide (or receptacle) from one level (depth)
• A deeper level or "step" will be considered another cut even if placed onto same slide
• A cut from a different sample placed onto the same slide as original sample will be considered a separate cut.

Tinctorial Staining:

A complete variety of common tinctorial stains are available for use. If a special stain (e.g. not currently offered) is required, we can accommodate most requests.

Immunohistochemical Staining:

Detection of cellular markers, techniques for research models, protocol development, validation/optimization.

UI Faculty Rates

Contact Information:

Christine Hochstedler, HT(ASCP)
Research Assistant III
319-335-8145, pager 5161
Email: christine-hochstedler@uiowa.edu

Equipment used in the Laboratory:

Cryostat A freezing microtome that allows the operator to create thin tissue sections from unfixed, fresh tissue.	Automated Tissue Processor Processes fixed tissues through a series of alcohols, xylenes, and paraffin wax, thus enabling the tissue to be thin sectioned for staining and microscopy.
Tissue Embedding Station With the use of metal molds, processed tissue can be placed into permanent paraffin wax blocks.	Rotary Microtome Operator creates thin tissue sections from fixed, processed paraffin-embedded tissues. Section may then be mounted on glass microscope slides for staining.
Automated Immunohistochemistry Stainer Processes up to 48 microscope slides through the complex antibody and detection steps of immunohistochemistry.

Publications: Meyerholz DK, Piester TL, McNamara AR, Sokolich JC, Jaskille AD, Orion KC, Zamba KD, Light TD. Pharmacologic modification to resuscitation fluid after thermal injury--is drotrecogin alfa the answer to arrest burn depth progression? Journal of Trauma, 2009, Nov;67(5):996-1003.

Wohlford-Lenane CL, Meyerholz DK, Perlman S, Zhou H, Tran D, Selsted ME, McCray PB Jr. Rhesus theta-defensin prevents death in a mouse model of severe acute respiratory syndrome coronavirus pulmonary disease. Journal of Virology, 2009, Nov;83(21):11385-90.

Dassie JP, Liu XY, Thomas GS, Whitaker RM, Thiel KW, Stockdale KR, Meyerholz DK, McCaffrey AP, McNamara JO 2nd, Giangrande PH. Systemic administration of optimized aptamer-siRNA chimeras promotes regression of PSMA-expressing tumors. Nature Biotechnology, 2009, Sep;27(9):839-49.

Lal G, Hashimi S, Smith BJ, Lynch CF, Zhang L, Robinson RA, Weigel RJ. Extracellular matrix 1 (ECM1) expression is a novel prognostic marker for poor long-term survival in breast cancer: a Hospital-based Cohort Study in Iowa. Annals of Surgical Oncology, 2009, Aug;16(8):2280-7.

McGill J, Meyerholz DK, Edsen-Moore M, Young B, Coleman RA, Schlueter AJ, Waldschmidt TJ, Cook RT, Legge KL. Fetal exposure to ethanol has long-term effects on the severity of influenza virus infections. Journal of Immunology, 2009, Jun 15;182(12):7803-8.

McNamara AR, Zamba KD, Sokolich JC, Jaskille AD, Light TD, Griffin MA, Meyerholz DK. Apoptosis is Differentially Regulated by Burn Severity and Dermal Location. Journal of Surgical Research, 2009, Feb 24.

Meyerholz DK, Rodgers J, Castilow EM, Varga SM. Alcian Blue and Pyronine Y histochemical stains permit assessment of multiple parameters in pulmonary disease models. Veterinary Pathology, 2009, Mar;46(2):325-8.

Ianzini F, Kosmacek EA, Nelson ES, Napoli E, Erenpreisa J, Kalejs M, Mackey MA. Activation of meiosis-specific genes is associated with depolyploidization of human tumor cells following radiation-induced mitotic catastrophe. Cancer Research, 2009, Mar 15;69(6):2296-304.

Meyerholz DK, Piester TL, Sokolich JC, Zamba GK, Light TD. Morphological parameters for assessment of burn severity in an acute burn injury rat model. International Journal of Experimental Pathology, 2009, Feb;90(1):26-33.

Meyerholz DK, Griffin MA, Castilow EM, Varga SM. Comparison of histochemical methods for murine eosinophil detection in an RSV vaccine-enhanced inflammation model. Toxicologic Pathology, 2009;37(2):249-55.

Itoh T, Iwashita S, Cohen, MB, Meyerholz DK, Linn, S. Ddb2 is a haploinsufficient tumor suppressor and controls spontaneous germ cell apoptosis. Human Molecular Genetics, 2007, 16(13):1578-1586.

Tigrani DY, Weydert JA. Immunohistochemical expression of osteopontin in epithelioid mesotheliomas and reactive mesothelial proliferations. American Journal of Clinical Pathology, 2007, Apr;127(4):580-4.

Thyagarajan B, Anderson KE, Folsom AR, Jacobs DR Jr, Lynch CF, Bargaje A, Khaliq W, Gross MD. No association between XRCC1 and XRCC3 gene polymorphisms and breast cancer risk: Iowa Women's Health Study. Cancer Detection and Prevention, 2006;30(4):313-21.

Visit the Comparative Pathology Laboratory