Specimen:

Plasma

Collection Medium:

Alternate
Collection Media:

Call laboratory for additional acceptable specimen collection 
containers.

Minimum:

5 mL whole blood from light green top tube or three light green top 
microtubes for pediatric patients

Testing
Schedule:

0700-1530 Monday through Friday.

Analytic Time:

4 days

Reference Range:

Non-reactive; positive with vaccine administration

Comments:

Part of initial diagnostic hepatitis profile.  Please indicate vaccine 
administration.  Immune status calculated.

Test
Limitations:

Nonspecific reaction may result from cross reactions in the immune 
"sandwich" complex.  Nonspecific reactions may include reactions with 
certain glycoproteins, such as concanavalin A, which interact with 
HBsAg.  Milliman and McMichael have shown that this hepatitis B binding 
substance is not antibody.  Any highly sensitive enzyme immunoassay 
system has the potential for nonspecific reaction in human serum or 
plasma.

Methodology:

Microparticle Enzymatic ImmunoAssay (MEIA)

Sample
Processing:

Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Label transport tube with two patient identifiers, date and time of 
collection.

Sample
Storage:

Refrigerate.

Transport
Instructions:

Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

86706