Plasma

Plasma Separator Tube

Call laboratory for additional acceptable specimen collection containers.

3 mL whole blood from light green top tube or TWO microtaines for pediatric patients

24 hrs/day, 7 days a week, including holidays.

1 hour (upon receipt in laboratory)

Non-reactive; positive with vaccine administration. Non-reactive samples have anti-HBs level less than 8.5 mIU/mL. Reactive samples have anti-HBs level greater than or equal to 11.5 mIU/mL. The result is indeterminate if the sample tests twice with anti-HBs results greater than or equal to 8.5 mIU/mL but less than 11.5 mIU/mL.

Part of initial diagnostic hepatitis profile or to verify immunity status in patients who have received the hepatitis B vaccine. Results include reactivity (REACTIVE or NON-REAC), immune status (IMMUNE or NON IMM OR INDETERM) and quantitative concentration. Anti-HBs can be formed following a hepatitis B infection or after hepatitis B vaccination.

New analytical immunoassay instituted April 5, 2010.

Assay is unaffected by icterus, hemolysis (Hb < 1600 mg/dL), and lipemia (triglycerides < 1500 mg/dL). In patients receiving high biotin doses
(> 5 mg/day) no sample should be taken until at least 8 hours after the last biotin administration. No false negative findings due to the high-dose hook effect are observed up to an anti-HBs concentration of 115,000 mIU/mL. In rare cases, interference due to extremely high titers of antibodies to analyte- specific antibodies, streptavidin, or ruthenium can occur.

For diagnostic purposes, the results should always be assessed in conjunction with the patient's medical history, clinical examination, and other findings. The assay is not intended for use in screening blood bank donors. A nonreactive test result does not exclude the possibility of exposure to or infection with hepatitis B. Individuals who have received blood component therapy, e.g., whole blood, plasma, immune globulin administration, during the previous 3 to 6 months may have a false reactive anti-HBs due to passive transfer of anti-HBs. Results from immunosuppressed individuals should be interpreted with caution.

Electrochemiluminescence Immunoassay (ECLIA)

Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Label transport tube with two patient identifiers, date and time of collection.

Refrigerate.

Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Transport in cooler with refrigerated coolant packs.

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