Hepatitis A Antibody, Total (IgG and IgM)
| Order Code: | HAVAB |
| Order Form: | Laboratory Requisition |
Specimen:
Plasma
Collection Medium:
 |
| Plasma Separator Tube |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood from light green top tube or TWO microtainers for
pediatric patients.
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
1 hour (upon receipt in laboratory)
Reference Range:
Negative
Interpretive Data:
The cutoff is set according to the WHO recommendation for vaccination.
Results obtained with the Elecsys Anti-HAV assay are resulted as
follows:
Non-reactive < 18.0 IU/L
Grayzone 18.0 ≤ IU/L < 22.0
Reactive ≥ 22.0 IU/L
If clinically indicated, patients who have samples that are resulted
as "Grayzone" should be re-tested in approximately two weeks.
Test
Limitations:
The assay is unaffected by icterus (bilirubin < 855 μmol/L or < 50
mg/dL), hemolysis (Hb < 0.623 mmol/L or < 100 mg/dL), lipemia
(Intralipid < 1500 mg/dL), and biotin < 205 nmol/L or < 50 ng/mL.
In patients receiving therapy with high biotin doses (i.e. > 5 mg/day), no sample should be taken until at least 8 hours after the last biotin administration.
As with all tests containing monoclonal mouse antibodies, erroneous findings may be obtained from samples taken from patients who have been treated with monoclonal mouse antibodies or have received them for diagnostic purposes. In rare cases, interference due to extremely high titers of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur. These effects are minimized by suitable test design.
In patients receiving therapy with high biotin doses (i.e. > 5 mg/day), no sample should be taken until at least 8 hours after the last biotin administration.
As with all tests containing monoclonal mouse antibodies, erroneous findings may be obtained from samples taken from patients who have been treated with monoclonal mouse antibodies or have received them for diagnostic purposes. In rare cases, interference due to extremely high titers of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur. These effects are minimized by suitable test design.
Methodology:
Electrichemiluminescent Immunoassay
Sample
Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Label transport tube with two patient identifiers, date and time of collection.
Aliquot plasma into labeled container and cap.
Label transport tube with two patient identifiers, date and time of collection.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
86708