Alpha-1-Antitrypsin Quantitation
Order Code: A1AT
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma Separator Tube
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in light green top tube or ONE microtainer
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
100-200 mg/dL A1AT is an acute phase reactant, therefore any inflammatory process may result in transient elevations of the patient's A-1-AT level.
Test Limitations:
Criterion: Recovery within plus or minus 10% of initial values. Icterus: No significant interference up to an I index of 60 (approximate conjugated and unconjugated bilirubin concentration: 60 mg/dL). Hemolysis: No significant interference up to an H index of 1000 (approximate hemoglobin concentration: 1000 mg/dL). Lipemia (Intralipid): No significant interference up to an L index of 890 (approximate triglycerides concentration: 1780 mg/dL). There is poor correlation between turbidity and triglycerides concentration. Rheumatoid factors less than 100 IU/mL do not interfere. No high dose hook effect is seen up to an alpha 1-antitrypsin concentration of 1200 mg/dL. Elevated estrogen levels (oral contraceptives; third trimester of pregnancy) give rise to false positive results. CRP and haptoglobin should therefore also be determined.* *Laurell CB, Kullander S, Thorell J. Effect of administration of a combined estrogen-progestin contraceptive on the level of individual plasma proteins. Scand J Lab Invest 1968;21:337-343.
Methodology:
Immunoturbidometric
Sample Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Sample Storage:
Refrigerate.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
82103