Hemoglobin Electrophoresis by HPLC
Order Code: HEOP
Order Form: Laboratory Requisition
Specimen:
Blood
Collection Medium:
Lavender top tube 3 mL (EDTA)
Minimum:
Full draw; 3.0 mL lavender top; (or fingerstick)
Testing Schedule:
Testing performed on Wednesday only; sample should be in the lab by Tuesday. Results usually reported on Thursday.
Reference Range:
Compatible with hemoglobin A, abnormal hemoglobins are quantitated by high performance liquid chromatography
Comments:
This test is appropriate for routine screening. Testing performed on Wednesday only. Sample should be in the lab by Tuesday. Pertinent clinical information should accompany the request and there should be a recent hematology profile. Path resident will interact with clinician on all quantitative hemoglobin orders. Peripheral smear morphology, RBC indicies, and HPLC results are correlated by the pathologist and a written narrative is reported by computer. Analysis cannot be done on patients transfused within the preceding 3 months since the presence of transfused cells may render the interpretation ambiguous. Results usually reported on Thursday.
Methodology:
High Pressure Liquid Chromatography and Wright Stain
Sample Processing:
Submit whole blood in original container.
Do Not Centrifuge.
Sample Storage:
Refrigerate.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
83021, 80500