Alpha Fetoprotein, Non-Pregnant, Plasma

Order Code:	AFP
Order Form:	Laboratory Requisition

Specimen:

Plasma

Collection Medium:

Plasma Separator Tube

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL whole blood in light green top tube or TWO microtainers for pediatric patients.

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

1 hour (upon receipt in laboratory)

Reference Range:

All units in ng/mL Age Male Female ------ ------------- ------------ 0-13 days 5,000 - 105,000 5,000 - 105,000 14-30 days 300 - 60,000 300 - 60,000 1 month 100 - 10,000 100 - 10,000 2 months 40 - 1,000 40 - 1,000 3 months 11 - 300 11 - 300 4 months 5 - 200 5 - 200 5 months 0 - 90 0 - 90 6-11 months 0 - 60 0 - 97 1 year 0 - 17 0 - 41 2 years 0 - 12 0 - 12 3+ years 0 - 9 0 - 9 Reference ranges updated 6/30/2011 by addition of pediatric reference ranges below 3 years old.

Comments:

Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Laboratory Requisition before shipping the specimen.

Test Limitations:

Hemolysis: No significant interference up to an H index of 2200 (approximate hemoglobin concentration: 2200 mg/dL).

Lipemia: No significant interference up to an L index of 1500 (approximate triglycerides concentration: 3000 mg/dL). Note there is poor correlation between turbidity and triglyceride concentration.

Icterus: No significant interference up to an I index of 65 (approximate conjugated and unconjugated bilirubin concentration: 65 mg/dL).

Methodology:

Electrochemiluminescence ImmunoAssay

Sample Processing:

Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.

Sample Storage:

Refrigerate.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

82105