Peak 5-8 mcg/mL; trough 1-2 mcg/mL. Peak levels: 45-75 min. After I.M. Dose, 15-30 min. After I.V. Dose. Trough levels: Not more than 30 min. Before next dose. Critical value: >15 mcg/mL

Sample Integrity Confirmation Icterus: No significant interference from bilirubin up to an I index of 60. Hemolysis: No significant interference from hemoglobin up to an H index of 1000. Lipemia: No significant interference from lipemia up to an L index of 2000. Sisomicin shows significant interference (greater than 50%) with the assay. The Gentamicin II System Pack assay performance has not been established with body fluids other than human serum and heparin or EDTA plasma. As with any assay employing mouse antibodies, the possibility exists for interference by human anti-mouse antibodies (HAMA) in the sample, which could cause falsely elevated results. The incidence of patients having antibodies to E. coli beta-galactosidase is extremely low. However, some samples containing such antibodies can result in artificially high gentamicin results that do not fit the clinical profile. If this occurs, contact Customer Technical Support at 1-800-428-2336 for assistance. A list of substances known to affect gentamicin levels in vivo is given by Young.10 No representation is made by Boehringer Mannheim regarding the completeness of this list or the accuracy of the information contained therein.