Follicle Stimulating Hormone (FSH)

Label Mnemonic:	FSH
Epic code:	LAB86
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	83001

Specimen(s):

Plasma

Collection Medium:

Plasma Separator Tube 4.5 mL

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL in light green top tube or TWO Microtainer^® devices

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

1 hour (upon receipt in laboratory)

Reference Range:

FEMALES - menstruating:
  Follicular phase        3.5 - 12.5 mIU/mL
  Ovulation phase         4.7 - 21.5 mIU/mL
  Luteal phase            1.7 - 7.7 mIU/mL
  Postmenopause          25.8 - 134.8 mIU/mL
MALES:                    1.5 - 12.4 mIU/mL

    AGE            MALE            FEMALE
1 - 3 years   0.2-1.5 mIU/mL   1.2-5.7 mIU/mL
4 - 8 years   0.5-1.6 mIU/mL   0.8-3.0 mIU/mL
Tanner I      0.7-3.1 mIU/mL   0.5-5.1 mIU/mL
Tanner II     1.1-6.9 mIU/mL   2.4-8.7 mIU/mL
Tanner III    1.8-6.2 mIU/mL   3.8-8.1 mIU/mL
Tanner IV     1.8-4.8 mIU/mL   1.1-9.6 mIU/mL
Tanner V      1.4-6.8 mIU/mL   2.0-7.6 mIU/mL

Interpretive Data:

Assay updated with increased tolerance to biotin on February 7, 2023.

Test Limitations:

The assay is unaffected by icterus (bilirubin is less than 64 
mg/dL), hemolysis (Hb is less than 1,000 mg/dL), lipemia (Intralipid 
is less than 1900 mg/dL) and biotin is less than 1200 ng/mL. (criterion: 
recovery within plus or minus 10% of initial value).

No interference was observed from rheumatoid factors up to a 
concentration of 2250 U/mL.

No high dose hook effect at FSH concentrations of up to 2000 mIU/mL.

In vitro tests were performed on 17 commonly used pharmaceuticals. No 
interference with the assay was found.

As with all tests containing monoclonal antibodies, erroneous findings 
may be obtained from samples taken from patients who have been treated 
with monoclonal mouse antibodies or who have received them for 
diagnostic purposes.

In rare cases, interference due to extremely high titers of antibodies 
to ruthenium can occur.

FSH contains additives which minimize these effects.

Extremely high titers of antibodies to streptavidin can occur in 
isolated cases and cause interference.

For diagnostic purposes, the FSH findings should always be assessed in 
conjunction with the patient's medical history, clinical examination 
and other findings.

Methodology:

Electrochemiluminescence Immunoassay

Sample Processing:

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Sample Storage:

Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

83001

See Additional Information:
Biotin Interference with Immunoassays