Estradiol (E2)
Order Code: EDIOL
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma Separator Tube
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood from light green top tube TWO microtainers
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
1 hour (upon receipt in laboratory)
Reference Range:
MALES 7 - 42 pg/mL FEMALES: Follicular Phase 12 - 166 pg/mL Ovulation Phase 85 - 498 pg/mL Luteal Phase 43 - 211 pg/mL Postmenopause <5 - 54 pg/mL Pregnancy 1st trimester 215 - 4300 pg/mL CHILDREN (1-10 YEARS) Boys <5 - 20 pg/mL Girls 6 - 27 pg/mL
Comments:
New analytical immunoassay with different reference ranges instituted 3/13/00 at 07:00.
Test Limitations:
The assay is unaffected by icterus (bilirubin is less than 66 mg/dL), hemolysis (Hb is less than 1.0 g/dL), lipemia (Intralipid is less than 1000 mg/dL) and biotin is less than 36 ng/mL. (criterion: recovery within plus or minus 10% of initial value). In patients receiving therapy with high biotin doses (i.e. is greater than 5 mg/day) no sample should be taken until at least 8 hours after the last biotin administration. No interference was observed from rheumatoid factors up to a concentration of 1200 U/mL. In vitro tests were performed on 18 commonly used pharmaceuticals. No interference with the assay was found. The risk of interference from potential immunological interactions between test components and rare sera has been minimized by the inclusion of suitable additives. In rare cases interference due to extremely high titers of antibodies to streptavidin and ruthenium can occur. Estradiol II contains additives which minimize these effects. For diagnostic purposes, the Estradiol II findings should always be assessed in conjunction with the patient's medical history, clinical examination and other findings.
Methodology:
Electrochemiluminescence
Sample Processing:
Aliquot plasma into labeled container and cap.
Centrifuge at 3000 RPM for 10 minutes.
Sample Storage:
Refrigerate.
Transport Instructions:
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
82670