Vitamin B12
| Label Mnemonic: | B12 |
| Epic code: | LAB67 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 82607 |
Specimen(s):
Plasma
Collection Medium:
 |
| Plasma Separator Tube 4.5 mL |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in light green top tube or TWO
Microtainer® devices.
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
1 hour (upon receipt in laboratory)
Reference Range:
Normal: 232 - 1245 pg/mL
Indeterminate: 150 - 231 pg/mL
Deficient: is less than 150 pg/mL
Indeterminate: 150 - 231 pg/mL
Deficient: is less than 150 pg/mL
Comments:
This assay may be significantly impacted by high-dose biotin (>5 mg
dose) taken within previous 12 hours. High concentrations of biotin
may lead to falsely increased results. These concentrations may be
found in patients taking over-the-counter supplements with biotin
content much higher than nutritional requirements for biotin.
Specimens should not be collected until at least 12 hours after the
last dose.
Refer to BD Microtainer® Tubes product sheet for detailed sample collection instructions.
Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Laboratory Requisition before shipping the specimen.
New analytical immunoassay with different reference range instituted on 11/26/2013.
Refer to BD Microtainer® Tubes product sheet for detailed sample collection instructions.
Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Laboratory Requisition before shipping the specimen.
New analytical immunoassay with different reference range instituted on 11/26/2013.
Test
Limitations:
The assay is unaffected by icterus (bilirubin is less than 65 mg/dL),
hemolysis (Hb is less than 1.0 g/dL), lipemia (triglycerides is less
than 1500 mg/dL) and biotin is less than 50 ng/mL. (criterion:
recovery within plus or minus 10% of initial value).
No interference was observed from rheumatoid factors up to a concentration of 1500 IU/mL.
In vitro tests were performed on 16 commonly used pharmaceuticals. No interference with the assay was found.
In rare cases interference due to extremely high titers of antibodies to streptavidin and ruthenium can occur.
This assay is designed to avoid interference due to anti-intrinsic factor antibodies.
For diagnostic purposes, the vitamin B12 findings should always be assessed in conjunction with the patient's medical history, clinical examination and other findings.
No interference was observed from rheumatoid factors up to a concentration of 1500 IU/mL.
In vitro tests were performed on 16 commonly used pharmaceuticals. No interference with the assay was found.
In rare cases interference due to extremely high titers of antibodies to streptavidin and ruthenium can occur.
This assay is designed to avoid interference due to anti-intrinsic factor antibodies.
For diagnostic purposes, the vitamin B12 findings should always be assessed in conjunction with the patient's medical history, clinical examination and other findings.
Methodology:
Electrochemiluminescence Immunoassay
Instructions:
Protect sample from light.
Sample
Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Protect specimen from light.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Protect specimen from light.
Sample
Storage:
Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
82607
See Additional Information:
Biotin Interference with Immunoassays
Biotin Interference with Immunoassays