|
University of Iowa Diagnostic Laboratories (UIDL) Test Directory 319-384-7212 (local) 1-866-844-2522 (toll free) |
|
| Alanine Aminotransferase (ALT) | Order Code: ALT
Order Form: Laboratory Requisition |
Specimen: |
Plasma | ||
Collection Medium: |
| ||
Alternate Collection Media: |
Call laboratory for additional acceptable specimen collection containers. | ||
Minimum: |
2 mL; light green top or 1 microtube for pediatric patients | ||
Testing Schedule: |
24 hrs/day, 7 days a week, including holidays. | ||
Analytic Time: |
1 hour (upon receipt in laboratory) | ||
Reference Range: |
Males 0-35 u/l; females 0-20 u/l | ||
Test Limitations: |
Criterion: Recovery within plus or minus 10% of initial value. Icterus: No significant interference up to an I index of 60 for conjugated and unconjugated bilirubin (approximate conjugated and unconjugated bilirubin concentration: 60 mg/dL). Hemolysis: No significant interference up to an H index of 200. RBC contamination may elevate results. Lipemia (Intralipid): No significant interference up to an L index of 500 (approximate triglycerides concentration: 1000 mg/dL). There is poor correlation between turbidity and triglycerides concentration. Lipemia may cause absorbance flagging as a result of an absorbance increase. Reference Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470-474. | ||
Methodology: |
Enzymatic | ||
Sample Processing: |
Centrifuge at 3000 RPM for 10 minutes. Aliquot plasma into labeled container and cap. | ||
Sample Storage: |
Refrigerate. | ||
Transport Instructions: |
Place specimen into zip-lock type bag, seal bag. Place requisition into outside pocket of bag. Transport in cooler with refrigerated coolant packs. | ||
CPT Code: |
84460 |
Updated: 08/22/2008