Alanine Aminotransferase (ALT), Plasma

Alanine Aminotransferase (ALT)

Order Code:	ALT
Order Form:	Laboratory Requisition

Specimen:

Plasma

Collection Medium:

Plasma Separator Tube

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL; light green top or 1 microtainer

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

1 hour (upon receipt in laboratory)

Reference Range:

Males 0-35 u/l; females 0-20 u/l Pediatric Normal Ranges: Age Male UI/L Female IU/L 1-30 days 1-25 2-25 31-365 days 4-35 3-30 1-3 years 5-30 5-30 4-6 years 5-20 5-25 7-9 years 5-25 5-25 10-18 years 5-30 5-20

Test Limitations:

Criterion: Recovery within plus or minus 10% of initial value. Icterus: No significant interference up to an I index of 60 for conjugated and unconjugated bilirubin (approximate conjugated and unconjugated bilirubin concentration: 60 mg/dL). Hemolysis: No significant interference up to an H index of 200. RBC contamination may elevate results. Lipemia (Intralipid): No significant interference up to an L index of 500 (approximate triglycerides concentration: 1000 mg/dL). There is poor correlation between turbidity and triglycerides concentration. Lipemia may cause absorbance flagging as a result of an absorbance increase. Reference Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470-474.

Methodology:

UV Testing

Sample Processing:

Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.

Sample Storage:

Refrigerate.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

84460

See also:
Alanine Aminotransferase-Other, Body Fluid (e.g., surgical drain, pericardial, peritoneal, peritoneal dialysis, pleural, or synovial)