Direct Antiglobulin Test
Label Mnemonic: | DAT |
Epic code: | LAB5548 |
Order form: | DeGowin Blood Center Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 86880 |
Specimen(s):
Blood
Collection Medium:
or | ||
Pink top tube 6 mL (K2-EDTA) | Lavender top tube 3 mL (EDTA) |
Minimum:
Adults - 2 mL
Pediatrics - 1 mL or EDTA Microtainer®
Pediatrics - 1 mL or EDTA Microtainer®
Rejection Criteria:
Specimen must be labeled with patient's first and last name and medical
record number. Specimens will be rejected if information is not on the
label when received.
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
2 hours (upon receipt in laboratory)
Reference Range:
Negative result means that no antibodies were detected on the patient's
red cells using polyspecific antiglobulin or anti IgG, depending on the
patient age. If the patient is greater than four months old, poly
specific antiglobulin is used. If the patient is less than four months
old, only IgG is used.
Comments:
Monospecific testing for IgG and C3 complement is automatically
performed when the polyspecific test is positive.
Elution performed per pathologist recommendation or clinician order.
Only monospecific testing of IgG will be performed on cord samples when mothers are alloimmunized, when mother's antibody status is unknown, or on samples from patients < 4 months old.
Elution performed per pathologist recommendation or clinician order.
Only monospecific testing of IgG will be performed on cord samples when mothers are alloimmunized, when mother's antibody status is unknown, or on samples from patients < 4 months old.
Methodology:
Tube test
Instructions:
If specimen is received in the laboratory greater than 24 hours from
time of collection and has not been refrigerated, it will be rejected.
Sample
Processing:
Invert tube gently several times to mix blood.
Label transport tube with patient last name, first name, identification number, date and time of collection.
Do Not Centrifuge.
Submit whole blood in original container.
Label transport tube with patient last name, first name, identification number, date and time of collection.
Do Not Centrifuge.
Submit whole blood in original container.
Sample
Storage:
Room temperature or refrigerate if stored overnight.
Transport
Instructions:
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Place specimen into Styrofoam container, seal container.
Ship at room temperature.
Place specimen into zip-lock type bag, seal bag.
Place specimen into Styrofoam container, seal container.
Ship at room temperature.
CPT Code:
86880