Specimen:

Blood

Collection Medium:

Minimum:

Adults - 2 mls
Pediatrics - 1 ml or EDTA microtainer

Rejection Criteria:

Specimen must be labeled with patient's first and last name and medical 
record number.  Specimens will be rejected if information is not on the 
label when received.

Testing
Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

2 hours (upon receipt in laboratory)

Reference Range:

Negative result means that no antibodies were detected on the patient's
red cells using polyspecific antiglobulin technique.

Comments:

Monospecific testing for IgG and C3 complement is automatically
performed when the polyspecific test is positive.

Elution performed per pathologist recommendation or clinician order.

Only monospecific testing of IgG will be performed on cord samples when 
mothers are alloimmunized or when mothers antibody status is unknown.

Methodology:

Tube test

Sample
Processing:

Invert tube gently several times to mix blood.
Label transport tube with patient last name, first name, identification 
number, date and time of collection.
Do Not Centrifuge.
Submit whole blood in original container.

Sample
Storage:

Room temperature or refrigerate if stored overnight.

Transport
Instructions:

Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Place specimen into styrofoam container, seal container.
Ship at ambient temperature.

Instructions:

If specimen will not be received in the laboratory within 24 hours of 
collection, please refrigerate specimen.

CPT Code:

86880