CMV IgG Antibody Detection
Label Mnemonic: | CMVG |
Epic code: | LAB937 |
Order form: | Laboratory Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 86644 |
Specimen(s):
Plasma
Collection Medium:
Plasma Separator Tube 4.5 mL |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3.0 mL whole blood from light green top tube or TWO Microtainer® devices.
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
3 hours (upon receipt in laboratory)
Reference Range:
Reference range and methodology changed effective 12/11/2012.
0.8 AI or less: Negative - No significant level of detectable CMV IgG antibody.
0.9-1.0 AI: Equivocal - Repeat testing in 10-14 days may be helpful.
1.1 AI or greater: Positive - IgG antibody to CMV detected, which may indicate a current or past CMV infection.
0.8 AI or less: Negative - No significant level of detectable CMV IgG antibody.
0.9-1.0 AI: Equivocal - Repeat testing in 10-14 days may be helpful.
1.1 AI or greater: Positive - IgG antibody to CMV detected, which may indicate a current or past CMV infection.
Comments:
The best evidence for current infection is a significant change on two
appropriately timed specimens, where both tests are done in the same
laboratory at the same time.
Test
Limitations:
Sera collected very early in the acute stage of disease may have
undetectable IgG levels. In immunocompromised patients, CMV serology
(IgG or IgM antibody titers) may not be reliable and may be misleading
in the diagnosis of acute or reactivation CMV disease. Positive results
from cord blood should be interpreted with caution. The presence of IgG
CMV antibodies in cord blood is usually the result of passive transfer
from mother to the fetus. A negative test, however, may be useful in
excluding current infection, but the most definitive diagnosis of CMV
infection requires viral culture. Positive test results may not be
valid in persons who have received blood transfusions or other blood
products within the past several months. Lack of significant increase
in antibody level does not exclude the possibility of CMV infection.
Methodology:
Multiplex Flow Immunoassay
Sample
Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells.
Separate plasma or serum into labeled container and cap.
Separate plasma or serum into labeled container and cap.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
86644