Specimen:

Peripheral Blood, Bone Marrow, or CSF

Collection Medium:

Alternate
Collection Media:

Green top tube (Na Heparin), Lavender top tube (EDTA)

Minimum:

Adult - Peripheral Blood: 10 mL; Bone Marrow:  2-4 mL

Pediatric - Peripheral Blood:  2-5 mL; Bone Marrow:  2-5 mL

CSF - Volume required is cell count dependent.  Provide as much CSF 
specimen as possible in the original CSF collection tube(s).

Testing
Schedule:

0800-1630 Monday through Friday.

Analytic Time:

2 days

Reference Range:

Antibodies routinely included are: CD2, CD3, CD7, CD10, CD13, CD14, 
CD19, CD33, CD34, CD38, CD45, CD46, CD56, CD64, CD117, HLA-DR, Kappa, 
Lambda, Glycophorin A, and Tdt as needed.

Additional antibodies are added as necessary for diagnosis after 
morphologic examination. The pathologist will provide an interpretative 
report.

Comments:

If after hours service is needed, contact UIHC operator at
319-356-1616 and have the Pathology Resident on-call paged.

Please print, complete and submit the Advance Beneficiary Notice 
(ABN) along with the Flow Cytometry Requisition before shipping the 
specimen.  Include pertinent clinical information on the requisition.

Additional antibodies may be necessary for diagnosis.

Test
Limitations:

Specimens with absolute lymphocyte counts of <100/mm3 will not be
tested.

Recent corticosteroid or chemotherapy may invalidate result.

Methodology:

Flow Cytometry-Whole Blood Lysis

Sample
Processing:

Relevant clinical information must be submitted with specimen in order 
to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as 
possible.

Sample
Storage:

Ambient or Room Temperature.

Transport
Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.
Recommend express mail or equivalent if not on courier service.

CPT Code:

Technical: 88184(x1), 88185(x18); Professional: 88189

See Additional Information:
Flow Cytometry Consultation Overview