Fluorescence In-Situ Hybridization (FISH-Tumors)

Order form:	Comprehensive Hematopathology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	88271 x # of probes 88275 x # of probes, 88291

Specimen(s):

Tumor Tissue

Minimum:

Specimen obtained aseptically according to your protocol. Collect specimen using sterile technique. DO NOT FREEZE OR CENTRIFUGE THE SPECIMEN. This analysis can be done on interphase nuclei or on metaphase cells. Contact the Cytogenetics Lab with questions.

Testing Schedule:

Specimens accepted in the lab Monday-Friday, 0800-1700.

Turn Around Time:

Preliminary results will be reported within 2-3 days and final results within 1-2 weeks.

Reference Range:

Male:  X and Y probe signals          Female:  two X probe signals
For autosomes, the reference range varies for each probe (set).  
Suitable for interphase and metaphase analysis.  Call lab for 
information.

Comments:

Epic code: CYT54

This test can be used for screening of sex chromosomes, for monosomies (eg., 5, 7, etc.), for trisomies (eg., +4, +8, +11, +12, +13, +14, +18, +19, +21, +22, etc.) or for detection of the gene fusion resulting from translocations in solid tumors.

If the desired FISH probe is not listed, call the laboratory.

Shivanand R. Patil Cytogenetics & Molecular Laboratory Testing Menu

Methodology:

Fluorescence In-Situ Hybridization (FISH)

Instructions:

Provide details of clinical information. If a specimen is 
collected over the weekend, please call (319) 356-1616 and ask the 
operator to page the cytogenetics on-call staff at pager #5525.

For billing and/or shipping questions, contact University of Iowa 
Diagnostic Laboratories at 1-866-844-2522 (toll free) or 319-384-7213 
(Fax).

Sample Processing:

Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Do Not Centrifuge.

Sample Storage:

Specimen cannot be frozen.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Ship at room temperature.

CPT Code:

88271 x # of probes
88275 x # of probes, 88291

See also:
Chromosomal Analysis, Bone Marrow (for acquired)