University of Iowa
Diagnostic Laboratories
(UIDL) Test Directory

319-384-7212 (local)
1-866-844-2522 (toll free)


Cholinesterase Order Code: CHE
Order Form: Laboratory Requisition
Specimen:
Serum
Collection Medium:
Red top tube
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection 
containers.
Minimum:
2 ml; red top or 0.4 ml microtube
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
3 hours (upon receipt in laboratory)
Reference Range:
3167 - 6333 u/l
Comments:
Availability:  As needed.
Test
Limitations:
Criterion: recovery ± 10% of initial values
Icterus: No significant interference up to an I index of 60 
(approximate unconjugated bilirubin concentration: 60 mg/dl).
Hemolysis: Significant interference. RBCs contain acetylcholinesterase,
thereby causing contamination.
Lipemia (Intralipid): No significant interference up to an L index of 
1000 (approximate triglycerides concentration: 2000 mg/dl). There is 
poor correlation between turbidity and triglycerides concentration.
Methodology:
Spectrophotometric
Sample
Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot serum into labeled container and cap.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
82480
 
See also:
Red Blood Cell Cholinesterase, Whole Blood

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Updated: 04/14/2004