University of Iowa
Diagnostic Laboratories
(UIDL) Test Directory

319-384-7212 (local)
1-866-844-2522 (toll free)


Cholesterol Order Code: CHOL
Order Form: Laboratory Requisition
Specimen:
Plasma
Collection Medium:
Plasma separator tube
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection 
containers.
Minimum:
2 ml whole blood from light green top tube or 1 light green microtube 
for pediatric patients
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
1 hour (upon receipt in laboratory)
Reference Range:
< 200 mg/dL - desirable
200-240 mg/dL - increase risk
above 240 mg/dL - significant increased risk
Comments:
Please print, complete and submit the Advance Beneficiary Notice 
(ABN) along with the Laboratory Requisition before shipping the 
specimen.
Test
Limitations:
The assay is unaffected by indexes of hemolysis <700 mg/dL, lipemia 
<1250 mg/dL and icterus <25 mg/dL.  For samples with bilirubin of 
greater than or equal to 25.0 mg/dL, the test will not be done and a 
comment will be added:  "Elevated bilirubin interferes with the 
analysis as done by present method."
Methodology:
Enzymatic colorimetric
Sample
Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Sample
Storage:
Refrigerate.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
82465

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Updated: 08/31/2007