Specimen:

Peripheral blood

Collection Medium:

Alternate
Collection Media:

Pink top tube (EDTA sprayed)

Minimum:

Adult: 5 mL whole blood (Lavender) or 7 mL whole blood (Pink)

Pediatric: 2 mL whole blood

Testing
Schedule:

0800-1630 Monday through Friday.

Analytic Time:

2 days

Reference Range:

Adult reference ranges for whole blood lysis method by flow cytometry:

T Cells (CD4)     34-62%     Absolute Counts:  298-2045/mm3

CD3 test is run as an internal quality assurance measure as directed by 
CDC guidelines. The results of this QA will not be charged.

Pediatric reference ranges will be provided with the interpretive
report.

Comments:

Maintain sample at room temperature; do not incubate or refrigerate.

Specimens with absolute lymphocyte counts of <100/mm3 will not be
tested.

Please print, complete and submit the Advance Beneficiary Notice 
(ABN) along with the Flow Cytometry Requisition before shipping the 
specimen.  Include pertinent clinical information on the reqisition.  
Recent corticosteroid or chemotherapy may invalidate result.

Methodology:

Flow Cytometry-Whole Blood Lysis

Sample
Processing:

Relevant clinical information must be submitted with specimen in order 
to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as 
possible.

Sample
Storage:

Ambient or Room Temperature.

Transport
Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.
Recommend express mail or equivalent if not on courier service.

CPT Code:

86361

See Additional Information:
Flow Cytometry Consultation Overview