Specimen:

Whole Blood

Collection Medium:

Minimum:

Full draw; any size lavender top or fingerstick.

Testing
Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

Routine turn around time is approximately 2.5 hours.

Reference Range:

                         Adults         Children (5-18 yrs)
                          (mm3)                (mm3)
Band Neutrophils          -                   -
Neutrophils           2188-7800/mm3       1700-7500/mm3
Eosinophils             40-390/mm3          40-650/mm3
Basophils               10-136/mm3           7-140/mm3
Monocytes              130-860/mm3          28-825/mm3
Lymphocytes            875-3300/mm3       1250-3380/mm3
Immature Granulocytes     -                   -

Comments:

A CBC must be ordered to perform a differential.  Hematology 
instruments are automated differential screening instruments that 
identify WBC's by their size and staining activity.  It performs a 
complete cell identification that includes neutrophils, lymphocytes, 
monocytes, basophils and eosinophils. The automated instruments count 
32,000 white blood cells and statistically provide a more accurate 
differential count than a manual differential.  All differentials will 
be analyzed on the instrument first.  If the results fail the screening 
criteria, a manual differential will be performed.  Pathologist 
approval is required to perform a manual differential on a specimen 
with an automated differential that passed the screening criteria.

Cases with significant abnormal cells may require a differential with 
narrative description and interpretation by the pathologist.  In these 
cases, results will be accompanied by the pathologist's report and 
charge.

Please print, complete and submit the Advance Beneficiary Notice 
(ABN) along with the Laboratory Requisition before shipping the 
specimen.

Please refer to the PowerPoint Presentation for further information.

Methodology:

Flow Cytometry
Auto Diff Cytochemical Staining
Manual Diff Wright's Stain

Sample
Processing:

Submit whole blood in original container.

Sample
Storage:

Refrigerate.

Transport
Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

85025

See Additional Information:
Hematology Pediatric Reference Ranges