Cancer Antigen 125, (Ca 125)
Label Mnemonic: | CA125 |
Epic code: | LAB155 |
Order form: | Laboratory Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 86304 |
Specimen(s):
Plasma
Collection Medium:
Plasma Separator Tube 4.5 mL |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in plasma separator tube or TWO
Microtainer® devices
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
1 hour (upon receipt in laboratory)
Reference Range:
0-34 U/mL
Comments:
This assay may be significantly impacted by high-dose biotin (>5 mg
dose) taken within previous 12 hours. High concentrations of biotin
may lead to falsely decreased results. These concentrations may be
found in patients taking over-the-counter supplements with biotin
content much higher than nutritional requirements for biotin.
Specimens should not be collected until at least 12 hours after the
last dose.
Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Laboratory Requisition before shipping the specimen.
Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Laboratory Requisition before shipping the specimen.
Test
Limitations:
As with all tests containing monoclonal antibodies, erroneous findings
may be obtained from samples taken from patients who have been treated
with monoclonal mouse antibodies (e.g., OC 125) or who have received
them for diagnostic purposes. In rare cases, interference due to
extremely high titers of antibodies to ruthenium can occur.
Elecsys CA125 II contains additives which minimize these effects.
Extremely high titers of antibodies to streptavidin can occur in
isolated cases and cause interference. For diagnostic purposes, the
Elecsys CA125 II finding should always be assessed in conjunction with
the patient's medical history, clinical examination and other
findings.
Hemolysis: No significant interference up to an H index of 3200 (approximate hemoglobin concentration: 3200 mg/dL).
Lipemia: No significant interference up to an L index of 2000 (approximate triglycerides concentration: 4000 mg/dL). Note there is poor correlation between turbidity and triglyceride concentration.
Icterus: No significant interference up to an I index of 66 (approximate conjugated and unconjugated bilirubin concentration: 66 mg/dL).
Hemolysis: No significant interference up to an H index of 3200 (approximate hemoglobin concentration: 3200 mg/dL).
Lipemia: No significant interference up to an L index of 2000 (approximate triglycerides concentration: 4000 mg/dL). Note there is poor correlation between turbidity and triglyceride concentration.
Icterus: No significant interference up to an I index of 66 (approximate conjugated and unconjugated bilirubin concentration: 66 mg/dL).
Methodology:
Electrochemiluminescence Immunoassay
Sample
Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Sample
Storage:
Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
86304
See Additional Information:
Biotin Interference with Immunoassays
Biotin Interference with Immunoassays