Salivary Gland Tumor Fusion Panel with Interpretation

Label Mnemonic:	SGTF
Epic code:	LAB8690
Order form:	Molecular Oncology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	81445

Specimen(s):

Formalin-fixed, paraffin embedded tissue block, or 10 unstained 4 micron-thick section on non-plus slides and one H&E stained section or Fine needle aspirate

Rejection Criteria:

Specimens that have been decalcified will not be accepted.

Testing Schedule:

Weekly

Turn Around Time:

21 days

Reference Range:

Fusion or mutation not detected.

Interpretive Data:

Detected
Not detected

Comments:

Tissue sections are reviewed by a pathologist. RNA is isolated from the selected area of the sample. Anchored multiplex PCR for a targeted next-generation sequencing is performed. The sequenced sample is a reverse transcription PCR-amplified fragenbt library in which each sample is uniquely identified by ligation of a short oligonucleotide barcode. The resultant sequence identifies the exons of the fusion transcript arising from the specific gene target and the partnet gene.

The panel target gene rearrangements of these gene targets: ETV6, EWSR1, HMGA2, HRAS, MAML2, MYB, MYBL1, NFIB, NTRK3, NUTM1, PLAG1, PRKD1, PRKD2, PRKD3.

Read more....

Methodology:

Massively paraellel Next Generation RNA sequencing by synthesis, semiconductor technology, Illumia

Sample Storage:

Room Temperature.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Recommend early AM overnight shipping or equivalent if not on courier service.
Protect block or slides from excessive heat and ship in cooled container during summer months.

CPT Code:

81445