Mitochondrial Antibodies (M2)
| Label Mnemonic: | AMA |
| Epic code: | LAB8746 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 83516 |
Specimen(s):
Serum
Collection Medium:
 |
| Red top tube 5 mL (Clot Activator) |
Minimum:
Preferred Minimum: 0.5 mL serum
Absolute Minimum: 0.4 mL serum
Absolute Minimum: 0.4 mL serum
Turn Around
Time:
1 day upon receipt at reference laboratory
Reference Range:
Negative: <0.1 Units
Borderline: 0.1-0.3 Units
Weakly positive: 0.4-0.9 Units
Positive: > or =1.0 Units
Reference values apply to all ages.
Borderline: 0.1-0.3 Units
Weakly positive: 0.4-0.9 Units
Positive: > or =1.0 Units
Reference values apply to all ages.
Interpretive Data:
Positive results for antimitochondrial antibody (AMA) of M2
specificity are highly specific for primary biliary cirrhosis (PBC),
and false-negative results are rare.
A positive result for AMA of M2 specificity in a patient with clinical features of PBC is virtually diagnostic for this disease.
A positive result for AMA of M2 specificity in a patient with clinical features of PBC is virtually diagnostic for this disease.
Comments:
Useful for: Establishing the diagnosis of primary biliary
cirrhosis.
Cautions: The level of antimitochondrial antibody (AMA) is not useful to indicate the stage or prognosis of the disease or for monitoring the course of disease. Positive results are found (infrequently) in patients with CREST (calcinosis, Raynaud's phenomenon, esophageal hypomotility, sclerodactyly, and telangiectasia) syndrome, relatives of patients with primary biliary cirrhosis and other autoimmune diseases.
Testing performed in the Immunology Antibody Laboratory of the antimitochondrial antibody (AMA)-M2 by EIA revealed a false-positive rate of <2% in normals and overall concordance compared with indirect immunofluorescence of 90% on sera from the Mayo primary biliary cirrhosis (PBC) Serum Bank. Ten discordant results were obtained (negative by EIA and positive by immunofluorescence assay). Seven of the 10 patients had no histologic evidence of PBC on liver biopsy.
Cautions: The level of antimitochondrial antibody (AMA) is not useful to indicate the stage or prognosis of the disease or for monitoring the course of disease. Positive results are found (infrequently) in patients with CREST (calcinosis, Raynaud's phenomenon, esophageal hypomotility, sclerodactyly, and telangiectasia) syndrome, relatives of patients with primary biliary cirrhosis and other autoimmune diseases.
Testing performed in the Immunology Antibody Laboratory of the antimitochondrial antibody (AMA)-M2 by EIA revealed a false-positive rate of <2% in normals and overall concordance compared with indirect immunofluorescence of 90% on sera from the Mayo primary biliary cirrhosis (PBC) Serum Bank. Ten discordant results were obtained (negative by EIA and positive by immunofluorescence assay). Seven of the 10 patients had no histologic evidence of PBC on liver biopsy.
Methodology:
Enzyme Immunoassay (EIA)
Sample
Processing:
Allow to clot completely at room temperature. Separate serum from cells ASAP.
Centrifuge within two hours of draw time.
Label transport tube with patient last name, first name, identification number, date and time of collection.
Transfer serum into new screw-capped plastic vial.
Centrifuge within two hours of draw time.
Label transport tube with patient last name, first name, identification number, date and time of collection.
Transfer serum into new screw-capped plastic vial.
Sample
Storage:
Store frozen.
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.
Place completed requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.
CPT Code:
83516