Saccharomyces cerevisiae Antibodies, IgG & IgA

Label Mnemonic:	ASCA
Epic code:	LAB8688
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	86671 x2

Specimen(s):

Serum

Collection Medium:

Red top tube 5 mL (Clot Activator)

Minimum:

Preferred Minimum: 0.5 mL serum
Absolute Minimum: 0.3 mL serum

Rejection Criteria:

Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Turn Around Time:

1-2 days upon receipt at reference laboratory

Reference Range:

S. cerevisiae Antibody, IgG
20.0 Units or less - Negative
20.1-24.9 Units - Equivocal
25.0 Units or greater - Positive

S. cerevisiae Antibody, IgA
20.0 Units or less - Negative
20.1-24.9 Units - Equivocal
25.0 Units or greater - Positive

Interpretive Data:

Saccharomyces cerevisiae IgG antibodies are found in 60-70% of Crohn disease (CD) patients and 10-15% of ulcerative colitis (UC) patients. Saccharomyces cerevisiae IgA antibodies are found in about 35% of CD patients but less than 1% in UC patients. Detection of both Saccharomyces IgG and IgA antibodies in the same serum specimen is highly specific for CD.

Comments:

This test may be a useful tool for distinguishing ulcerative colitis (UC) from Crohn disease (CD) in patients with suspected inflammatory bowel disease.

Methodology:

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Sample Processing:

Allow to clot completely at room temperature. Separate serum from cells ASAP.
Centrifuge within two hours of draw time.
Label transport tube with patient last name, first name, identification number, date and time of collection.
Transfer serum into new screw-capped plastic vial.
Hemolyzed samples are not acceptable.

Sample Storage:

Store frozen.

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.

CPT Code:

86671 x2