F-Actin (Smooth Muscle) Antibody, IgG with Reflex to IgG Titer
Label Mnemonic: | ACTIN |
Epic code: | LAB8686 |
Order form: | Laboratory Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 83516; if titer performed, 86256 |
Specimen(s):
Serum
Collection Medium:
Red top tube 5 mL (Clot Activator) |
Minimum:
Preferred Minimum: 1 mL serum
Absolute Minimum: 0.25 mL serum
Absolute Minimum: 0.25 mL serum
Rejection Criteria:
Plasma. Contaminated, heat-inactivated, hemolyzed, or lipemic
specimens.
Turn Around
Time:
1-5 days upon receipt at reference laboratory
Reference Range:
F-Actin (Smooth Muscle) Ab, IgG by ELISA 19 Units or less Negative 20-30 Units Weak Positive - Suggest repeat testing in two to three weeks with fresh specimen. 31 Units or greater Positive - Suggestive of autoimmune hepatitis or chronic active hepatitis. Smooth Muscle Antibody, IgG Titer Less than 1:20 Negative - No Antibody detected. 1:20-1:80 Weak Positive - Suggest repeat testing in two to three weeks with fresh specimen. 1:160 or greater Positive - Suggestive of autoimmune hepatitis or chronic active hepatitis.
Interpretive Data:
Refer to report.
Comments:
In viral hepatitis the titers are generally less than 1:80 and are
transient. The titers in primary biliary cirrhosis are also low,
ranging from 1:20 to 1:40.
If F-Actin is 20 Units or greater, then Smooth Muscle Ab, IgG IFA titer will be added. Additional charges apply.
If F-Actin is 20 Units or greater, then Smooth Muscle Ab, IgG IFA titer will be added. Additional charges apply.
Methodology:
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative
Indirect Fluorescent Antibody
Sample
Processing:
Allow to clot completely at room temperature. Separate serum from cells ASAP.
Centrifuge within two hours of draw time.
Label transport tube with patient last name, first name, identification number, date and time of collection.
Transfer serum into new screw-capped plastic vial.
Hemolyzed samples are not acceptable.
Centrifuge within two hours of draw time.
Label transport tube with patient last name, first name, identification number, date and time of collection.
Transfer serum into new screw-capped plastic vial.
Hemolyzed samples are not acceptable.
Sample
Storage:
Store frozen.
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.
Place completed requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.
CPT Code:
83516; if titer performed, 86256