JAK2 Exon 12 Mutation
| Label Mnemonic: | JAK2EX12 |
| Epic code: | LAB8052 |
| Order form: | Comprehensive Hematopathology Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 81279 |
Specimen(s):
Blood
Collection Medium:
 |
| Pink top tube 6 mL (K2-EDTA) |
Minimum:
5 mL whole blood in pink top (EDTA) tube
Rejection Criteria:
Serum. Frozen specimens. Clotted or grossly hemolyzed specimens.
Specimens collected in anticoagulants other than EDTA.
Turn Around
Time:
7-10 days upon receipt
Interpretive Data:
Refer to report.
Comments:
May be useful in cases with suspicion of polycythemia vera and
negative JAK2 V617F mutation status.
Methodology:
Massively parallel Next Generation DNA sequencing by synthesis, semiconductor technology, Ion Personal Genomics Machine
Sample
Processing:
Label transport tube with two patient identifiers, date and time of collection.
Patient's age and sex is required on requisition for processing.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Lavender tube is sent as whole blood, no processing necessary.
Do Not Centrifuge.
Patient's age and sex is required on requisition for processing.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Lavender tube is sent as whole blood, no processing necessary.
Do Not Centrifuge.
Sample
Storage:
Room temperature for up to 24 hours, then refrigerate the whole blood if it is necessary to be held overnight, weekends, or holidays.
Transport
Instructions:
Place requisition into outside pocket of bag.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship refrigerated.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship refrigerated.
CPT Code:
81279